2as9

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(New page: 200px<br /><applet load="2as9" size="350" color="white" frame="true" align="right" spinBox="true" caption="2as9, resolution 1.70&Aring;" /> '''Functional and struc...)
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[[Image:2as9.gif|left|200px]]<br /><applet load="2as9" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2as9, resolution 1.70&Aring;" />
 
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'''Functional and structural characterization of Spl proteases from staphylococcus aureus'''<br />
 
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==Overview==
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==Functional and structural characterization of Spl proteases from staphylococcus aureus==
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Staphylococcus aureus is the major cause of nosocomial infections, world-wide, with increasing prevalence of community-acquired diseases. The, recent dramatic increase in multi-antibiotic resistance, including, resistance to the last-resort drug, vancomycin, together with the lack of, an effective vaccine highlight the need for better understanding of, S.aureus pathogenicity. Comparative analysis of available bacterial, genomes allows for the identification of previously uncharacterized, S.aureus genes with potential roles in pathogenicity. A good example is a, cluster of six serine protease-like (spl) genes encompassed in one operon, which encode for putative proteases with similarity to staphylococcal, glutamylendopeptidase (V8 protease). Here, we describe an efficient, expression system for the production of recombinant SplB and SplC, proteases in Escherichia coli, together with structural and functional, characterization of the purified enzymes. A unique mechanism of cytoplasm, protection against activity of misdirected SplB was uncovered. Apparently, the co-translated signal peptide maintains protease latency until it is, cleaved by the signal peptidase during protein secretion. Furthermore, the, crystal structure of the SplC protease revealed a fold resembling that of, the V8 protease and epidermolytic toxins. Arrangement of the active site, cleft and substrate-binding pocket of SplC explains the mechanism of, enzyme latency and suggests that some Spl proteases possess restricted, substrate specificity similar to that of the V8 protease and epidermolytic, toxins.
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<StructureSection load='2as9' size='340' side='right'caption='[[2as9]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2as9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AS9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AS9 FirstGlance]. <br>
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2AS9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AS9 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2as9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2as9 OCA], [https://pdbe.org/2as9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2as9 RCSB], [https://www.ebi.ac.uk/pdbsum/2as9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2as9 ProSAT]</span></td></tr>
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Functional and structural characterization of Spl proteases from Staphylococcus aureus., Popowicz GM, Dubin G, Stec-Niemczyk J, Czarny A, Dubin A, Potempa J, Holak TA, J Mol Biol. 2006 Apr 21;358(1):270-9. Epub 2006 Feb 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16516230 16516230]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/SPLC_STAA8 SPLC_STAA8]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/as/2as9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2as9 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Czarny, A.]]
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[[Category: Czarny A]]
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[[Category: Dubin, A.]]
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[[Category: Dubin A]]
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[[Category: Dubin, G.]]
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[[Category: Dubin G]]
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[[Category: Holak, T.A.]]
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[[Category: Holak TA]]
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[[Category: Popowicz, G.M.]]
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[[Category: Popowicz GM]]
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[[Category: Potempa, J.]]
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[[Category: Potempa J]]
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[[Category: Stec-Niemczyk, J.]]
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[[Category: Stec-Niemczyk J]]
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[[Category: ZN]]
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[[Category: serine protease]]
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[[Category: staphylococcus aureus]]
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[[Category: trypsin-like fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:08:52 2008''
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Current revision

Functional and structural characterization of Spl proteases from staphylococcus aureus

PDB ID 2as9

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