2avw

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(New page: 200px<br /><applet load="2avw" size="350" color="white" frame="true" align="right" spinBox="true" caption="2avw, resolution 2.0&Aring;" /> '''Crystal structure of ...)
Current revision (09:14, 14 February 2024) (edit) (undo)
 
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[[Image:2avw.gif|left|200px]]<br /><applet load="2avw" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2avw, resolution 2.0&Aring;" />
 
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'''Crystal structure of monoclinic form of streptococcus Mac-1'''<br />
 
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==Overview==
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==Crystal structure of monoclinic form of streptococcus Mac-1==
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Group A Streptococcus secretes cysteine proteases named Mac-1 and Mac-2, that mediate host immune evasion by targeting both IgG and Fc receptors., Here, we report the crystal structures of Mac-1 and its catalytically, inactive C94A mutant in two different crystal forms. Despite the lack of, sequence homology, Mac-1 adopts the canonical papain fold. Alanine, mutations at the active site confirmed the critical residues involved in a, papain-like catalytic mechanism. Mac-1 forms a symmetric dimer in both, crystal forms and displays the unique dimer interface among papain, superfamily members. Mutations at the dimer interface resulted in a, significant reduction in IgG binding and catalysis, suggesting that the, dimer contributes to both IgG specificity and enzyme cooperativity. A, tunnel observed at the dimer interface constitutes a target for designing, potential Mac-1-specific antimicrobial agents. The structures also offer, insight into the functional difference between Mac-1 and Mac-2.
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<StructureSection load='2avw' size='340' side='right'caption='[[2avw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2avw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_MGAS5005 Streptococcus pyogenes MGAS5005]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AVW FirstGlance]. <br>
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2AVW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes_mgas315 Streptococcus pyogenes mgas315] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AVW OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2avw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2avw OCA], [https://pdbe.org/2avw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2avw RCSB], [https://www.ebi.ac.uk/pdbsum/2avw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2avw ProSAT]</span></td></tr>
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Crystal structure of group A streptococcus Mac-1: insight into dimer-mediated specificity for recognition of human IgG., Agniswamy J, Nagiec MJ, Liu M, Schuck P, Musser JM, Sun PD, Structure. 2006 Feb;14(2):225-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16472742 16472742]
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</table>
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[[Category: Single protein]]
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== Function ==
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[[Category: Streptococcus pyogenes mgas315]]
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[https://www.uniprot.org/uniprot/Q7DAM2_STRP1 Q7DAM2_STRP1]
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[[Category: Agniswamy, J.]]
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== Evolutionary Conservation ==
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[[Category: Liu, M.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Musser, J.M.]]
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Check<jmol>
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[[Category: Nagiec, M.J.]]
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<jmolCheckbox>
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[[Category: Schuck, P.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/av/2avw_consurf.spt"</scriptWhenChecked>
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[[Category: Sun, P.D.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: GOL]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: SO4]]
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</jmolCheckbox>
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[[Category: mac-1]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2avw ConSurf].
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<div style="clear:both"></div>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:11:01 2008''
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Streptococcus pyogenes MGAS5005]]
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[[Category: Agniswamy J]]
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[[Category: Liu M]]
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[[Category: Musser JM]]
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[[Category: Nagiec MJ]]
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[[Category: Schuck P]]
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[[Category: Sun PD]]

Current revision

Crystal structure of monoclinic form of streptococcus Mac-1

PDB ID 2avw

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