2awy

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(New page: 200px<br /><applet load="2awy" size="350" color="white" frame="true" align="right" spinBox="true" caption="2awy, resolution 2.100&Aring;" /> '''met-DcrH-Hr'''<br /...)
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[[Image:2awy.gif|left|200px]]<br /><applet load="2awy" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2awy, resolution 2.100&Aring;" />
 
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'''met-DcrH-Hr'''<br />
 
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==Overview==
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==met-DcrH-Hr==
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The methyl-accepting chemotaxis protein, DcrH, from the anaerobic, sulfate-reducing bacterium, Desulfovibrio vulgaris (Hildenborough), has a, hemerythrin-like domain, DcrH-Hr, at its C terminus. DcrH-Hr was, previously shown to contain a diiron site that binds O2, suggesting an, O2-sensing function. X-ray crystal structures of diferric (met-), azido-diferric (azidomet-), and diferrous (deoxy-) DcrH-Hr reveal a, "substrate tunnel" distinct from that in invertebrate hemerythrins. This, tunnel is proposed to facilitate the rapid autoxidation of oxy-DcrH-Hr and, suggests that sensing is triggered by O2 binding and subsequent oxidation, of the diferrous active site. The N-terminal loop of DcrH-Hr is highly, ordered in both met- and azidomet-DcrH-Hr but is disordered in, deoxy-DcrH-Hr. These redox-dependent conformational differences presumably, transduce the sensory signal of DcrH-Hr to the neighboring methylation, domain in the full-length receptor. Given the putative cytoplasmic, localization of its Hr-like O2-sensing domain, DcrH is proposed to serve a, role in negative aerotaxis (anaerotaxis).
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<StructureSection load='2awy' size='340' side='right'caption='[[2awy]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2awy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AWY FirstGlance]. <br>
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2AWY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=FEO:'>FEO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AWY OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FEO:MU-OXO-DIIRON'>FEO</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2awy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2awy OCA], [https://pdbe.org/2awy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2awy RCSB], [https://www.ebi.ac.uk/pdbsum/2awy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2awy ProSAT]</span></td></tr>
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Structural basis for O2 sensing by the hemerythrin-like domain of a bacterial chemotaxis protein: substrate tunnel and fluxional N terminus., Isaza CE, Silaghi-Dumitrescu R, Iyer RB, Kurtz DM Jr, Chan MK, Biochemistry. 2006 Aug 1;45(30):9023-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16866347 16866347]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9REU3_DESVU Q9REU3_DESVU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aw/2awy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2awy ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Desulfovibrio vulgaris]]
[[Category: Desulfovibrio vulgaris]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chan, M.K.]]
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[[Category: Chan MK]]
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[[Category: Isaza, C.E.]]
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[[Category: Isaza CE]]
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[[Category: Iyer, R.B.]]
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[[Category: Iyer RB]]
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[[Category: Kurtz, D.M.]]
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[[Category: Kurtz DM]]
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[[Category: Silaghi-Dumitrescu, R.]]
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[[Category: Silaghi-Dumitrescu R]]
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[[Category: CA]]
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[[Category: CL]]
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[[Category: FEO]]
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[[Category: hemerythrin-like oxygen sensor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 18:11:48 2008''
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Current revision

met-DcrH-Hr

PDB ID 2awy

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