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| - | [[Image:2l5a.png|left|200px]] | |
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| - | <!-- | + | ==Structural basis for recognition of centromere specific histone H3 variant by nonhistone Scm3== |
| - | The line below this paragraph, containing "STRUCTURE_2l5a", creates the "Structure Box" on the page.
| + | <StructureSection load='2l5a' size='340' side='right'caption='[[2l5a]]' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[2l5a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L5A FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l5a OCA], [https://pdbe.org/2l5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l5a RCSB], [https://www.ebi.ac.uk/pdbsum/2l5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l5a ProSAT]</span></td></tr> |
| - | {{STRUCTURE_2l5a| PDB=2l5a | SCENE= }}
| + | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/SCM3_YEAST SCM3_YEAST] Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres.[UniProtKB:Q8NCD3][https://www.uniprot.org/uniprot/CENPA_YEAST CENPA_YEAST] Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. Required for functional chromatin architecture at the yeast 2-micron circle partitioning locus and promotes equal plasmid segregation.<ref>PMID:7698647</ref> <ref>PMID:9741625</ref> <ref>PMID:9584087</ref> <ref>PMID:10454560</ref> <ref>PMID:11063678</ref> <ref>PMID:10891506</ref> <ref>PMID:10499801</ref> <ref>PMID:11606525</ref> <ref>PMID:15590827</ref> <ref>PMID:16207811</ref> <ref>PMID:16966420</ref> [https://www.uniprot.org/uniprot/H4_YEAST H4_YEAST] |
| | | | |
| - | ===Structural basis for recognition of centromere specific histone H3 variant by nonhistone Scm3=== | + | ==See Also== |
| - | | + | *[[Histone 3D structures|Histone 3D structures]] |
| - | | + | == References == |
| - | <!--
| + | <references/> |
| - | The line below this paragraph, {{ABSTRACT_PUBMED_21412236}}, adds the Publication Abstract to the page
| + | __TOC__ |
| - | (as it appears on PubMed at http://www.pubmed.gov), where 21412236 is the PubMed ID number.
| + | </StructureSection> |
| - | -->
| + | [[Category: Large Structures]] |
| - | {{ABSTRACT_PUBMED_21412236}}
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | [[2l5a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L5A OCA]. | + | |
| - | | + | |
| - | ==Reference== | + | |
| - | <ref group="xtra">PMID:021412236</ref><references group="xtra"/> | + | |
| | [[Category: Saccharomyces cerevisiae]] | | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Bai, Y.]] | + | [[Category: Bai Y]] |
| - | [[Category: Feng, H.]] | + | [[Category: Feng H]] |
| - | [[Category: Ghirlando, R.]] | + | [[Category: Ghirlando R]] |
| - | [[Category: Hu, K.]] | + | [[Category: Hu K]] |
| - | [[Category: Jenkins, L.]] | + | [[Category: Jenkins L]] |
| - | [[Category: Tjandra, N.]] | + | [[Category: Tjandra N]] |
| - | [[Category: Wu, C.]] | + | [[Category: Wu C]] |
| - | [[Category: Xiao, H.]] | + | [[Category: Xiao H]] |
| - | [[Category: Zhou, B.]] | + | [[Category: Zhou B]] |
| - | [[Category: Zhou, Z.]] | + | [[Category: Zhou Z]] |
| - | [[Category: Zwolak, A.]] | + | [[Category: Zwolak A]] |
| Structural highlights
Function
SCM3_YEAST Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres.[UniProtKB:Q8NCD3]CENPA_YEAST Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. Required for functional chromatin architecture at the yeast 2-micron circle partitioning locus and promotes equal plasmid segregation.[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] H4_YEAST
See Also
References
- ↑ Stoler S, Keith KC, Curnick KE, Fitzgerald-Hayes M. A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosis. Genes Dev. 1995 Mar 1;9(5):573-86. PMID:7698647
- ↑ Meluh PB, Yang P, Glowczewski L, Koshland D, Smith MM. Cse4p is a component of the core centromere of Saccharomyces cerevisiae. Cell. 1998 Sep 4;94(5):607-13. PMID:9741625
- ↑ Baker RE, Harris K, Zhang K. Mutations synthetically lethal with cep1 target S. cerevisiae kinetochore components. Genetics. 1998 May;149(1):73-85. PMID:9584087
- ↑ Keith KC, Baker RE, Chen Y, Harris K, Stoler S, Fitzgerald-Hayes M. Analysis of primary structural determinants that distinguish the centromere-specific function of histone variant Cse4p from histone H3. Mol Cell Biol. 1999 Sep;19(9):6130-9. PMID:10454560
- ↑ Keith KC, Fitzgerald-Hayes M. CSE4 genetically interacts with the Saccharomyces cerevisiae centromere DNA elements CDE I and CDE II but not CDE III. Implications for the path of the centromere dna around a cse4p variant nucleosome. Genetics. 2000 Nov;156(3):973-81. PMID:11063678
- ↑ Glowczewski L, Yang P, Kalashnikova T, Santisteban MS, Smith MM. Histone-histone interactions and centromere function. Mol Cell Biol. 2000 Aug;20(15):5700-11. PMID:10891506
- ↑ Tanaka T, Cosma MP, Wirth K, Nasmyth K. Identification of cohesin association sites at centromeres and along chromosome arms. Cell. 1999 Sep 17;98(6):847-58. PMID:10499801
- ↑ Biggins S, Bhalla N, Chang A, Smith DL, Murray AW. Genes involved in sister chromatid separation and segregation in the budding yeast Saccharomyces cerevisiae. Genetics. 2001 Oct;159(2):453-70. PMID:11606525
- ↑ Morey L, Barnes K, Chen Y, Fitzgerald-Hayes M, Baker RE. The histone fold domain of Cse4 is sufficient for CEN targeting and propagation of active centromeres in budding yeast. Eukaryot Cell. 2004 Dec;3(6):1533-43. PMID:15590827 doi:http://dx.doi.org/10.1128/EC.3.6.1533-1543.2004
- ↑ Collins KA, Castillo AR, Tatsutani SY, Biggins S. De novo kinetochore assembly requires the centromeric histone H3 variant. Mol Biol Cell. 2005 Dec;16(12):5649-60. Epub 2005 Oct 5. PMID:16207811 doi:http://dx.doi.org/10.1091/mbc.E05-08-0771
- ↑ Hajra S, Ghosh SK, Jayaram M. The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-microm circle partitioning locus and promotes equal plasmid segregation. J Cell Biol. 2006 Sep 11;174(6):779-90. PMID:16966420 doi:http://dx.doi.org/jcb.200603042
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