3nt7

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[[Image:3nt7.jpg|left|200px]]
 
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==Crystal Structure of Vaccinia Virus Uracil DNA Glycosylase R187V Mutant==
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The line below this paragraph, containing "STRUCTURE_3nt7", creates the "Structure Box" on the page.
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<StructureSection load='3nt7' size='340' side='right'caption='[[3nt7]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3nt7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus_Western_Reserve Vaccinia virus Western Reserve]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NT7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NT7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3nt7| PDB=3nt7 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nt7 OCA], [https://pdbe.org/3nt7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nt7 RCSB], [https://www.ebi.ac.uk/pdbsum/3nt7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nt7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UNG_VACCW UNG_VACCW] Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. Also part of a heterodimeric processivity factor which potentiates the DNA polymerase activity. Binds to DNA (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Genome replication is inefficient without processivity factors, which tether DNA polymerases to their templates. The vaccinia virus DNA polymerase E9 requires two viral proteins, A20 and D4, for processive DNA synthesis, yet the mechanism of how this tricomplex functions is unknown. This study confirms that these three proteins are necessary and sufficient for processivity, and it focuses on the role of D4, which also functions as a uracil DNA glycosylase (UDG) repair enzyme. A series of D4 mutants was generated to discover which sites are important for processivity. Three point mutants (K126V, K160V, and R187V) which did not function in processive DNA synthesis, though they retained UDG catalytic activity, were identified. The mutants were able to compete with wild-type D4 in processivity assays and retained binding to both A20 and DNA. The crystal structure of R187V was resolved and revealed that the local charge distribution around the substituted residue is altered. However, the mutant protein was shown to have no major structural distortions. This suggests that the positive charges of residues 126, 160, and 187 are required for D4 to function in processive DNA synthesis. Consistent with this is the ability of the conserved mutant K126R to function in processivity. These mutants may help unlock the mechanism by which D4 contributes to processive DNA synthesis.
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===Crystal Structure of Vaccinia Virus Uracil DNA Glycosylase R187V Mutant===
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Vaccinia virus D4 mutants defective in processive DNA synthesis retain binding to A20 and DNA.,Druck Shudofsky AM, Silverman JE, Chattopadhyay D, Ricciardi RP J Virol. 2010 Dec;84(23):12325-35. Epub 2010 Sep 22. PMID:20861259<ref>PMID:20861259</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3nt7" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20861259}}, adds the Publication Abstract to the page
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*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20861259 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20861259}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3nt7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NT7 OCA].
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[[Category: Vaccinia virus Western Reserve]]
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[[Category: Chattopadhyay D]]
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==Reference==
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<ref group="xtra">PMID:020861259</ref><references group="xtra"/>
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[[Category: Uracil-DNA glycosylase]]
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[[Category: Vaccinia virus]]
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[[Category: Chattopadhyay, D.]]
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Current revision

Crystal Structure of Vaccinia Virus Uracil DNA Glycosylase R187V Mutant

PDB ID 3nt7

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