3aj3

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[[Image:3aj3.jpg|left|200px]]
 
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==Crystal structure of selenomethionine substituted 4-pyridoxolactonase from Mesorhizobium loti==
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The line below this paragraph, containing "STRUCTURE_3aj3", creates the "Structure Box" on the page.
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<StructureSection load='3aj3' size='340' side='right'caption='[[3aj3]], [[Resolution|resolution]] 1.58&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3aj3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AJ3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.577&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3aj3| PDB=3aj3 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aj3 OCA], [https://pdbe.org/3aj3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aj3 RCSB], [https://www.ebi.ac.uk/pdbsum/3aj3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aj3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PDLA_RHILO PDLA_RHILO] Involved in the degradation of pyridoxine or pyridoxamine (free, phosphate-unbound, forms of vitamin B6). Hydrolyzes 4-pyridoxolactone to 4-pyridoxic acid. Has lower activity toward N-hexanoyl-D,L-homoserine lactone, but is not active toward 5-pyridoxolactone and gamma-butyrolactone.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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4-Pyridoxolactonase from Mesorhizobium loti catalyzes the zinc-dependent lactone-ring hydrolysis of 4-pyridoxolactone (4PAL) to 4-pyridoxic acid (4PA) in vitamin B6 degradation pathway I. The crystal structures of 4-pyridoxolactonase and its complex with 5-pyridoxolactone (5PAL; the competitive inhibitor) were determined. The overall structure was an alphabeta/betaalpha sandwich fold, and two zinc ions were coordinated. This strongly suggested that the enzyme belongs to subclass B3 of the class B beta-lactamases. In the complex structure, the carbonyl group of 5PAL pointed away from the active site, revealing why it acts as a competitive inhibitor. Based on docking simulation with 4PAL, 4PA and a reaction intermediate, 4-pyridoxolactonase probably catalyzes the reaction through a subclass B2-like mechanism, not the subclass B3 mechanism.
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===Crystal structure of selenomethionine substituted 4-pyridoxolactonase from Mesorhizobium loti===
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Structure of 4-pyridoxolactonase from Mesorhizobium loti.,Kobayashi J, Yoshikane Y, Yagi T, Baba S, Mizutani K, Takahashi N, Mikami B Acta Crystallogr F Struct Biol Commun. 2014 Apr;70(Pt 4):424-32. doi:, 10.1107/S2053230X14003926. Epub 2014 Mar 25. PMID:24699732<ref>PMID:24699732</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[3aj3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AJ3 OCA].
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<div class="pdbe-citations 3aj3" style="background-color:#fffaf0;"></div>
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[[Category: 4-pyridoxolactonase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mesorhizobium loti]]
[[Category: Mesorhizobium loti]]
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[[Category: Baba, S.]]
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[[Category: Baba S]]
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[[Category: Kobayashi, J.]]
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[[Category: Kobayashi J]]
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[[Category: Mikami, B.]]
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[[Category: Mikami B]]
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[[Category: Yagi, T.]]
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[[Category: Yagi T]]
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[[Category: Yoshikane, Y.]]
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[[Category: Yoshikane Y]]

Current revision

Crystal structure of selenomethionine substituted 4-pyridoxolactonase from Mesorhizobium loti

PDB ID 3aj3

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