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2zq8

From Proteopedia

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[[Image:2zq8.png|left|200px]]
 
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==Apo structure of class a beta-lactamase Toho-1 R274N/R276N double mutant==
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The line below this paragraph, containing "STRUCTURE_2zq8", creates the "Structure Box" on the page.
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<StructureSection load='2zq8' size='340' side='right'caption='[[2zq8]], [[Resolution|resolution]] 1.03&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2zq8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZQ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZQ8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.03&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2zq8| PDB=2zq8 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zq8 OCA], [https://pdbe.org/2zq8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zq8 RCSB], [https://www.ebi.ac.uk/pdbsum/2zq8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zq8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BLT1_ECOLX BLT1_ECOLX] Has strong cefotaxime-hydrolyzing activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zq/2zq8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zq8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The beta-lactamase Toho-1 exhibits a strong tendency to form merohedrally twinned crystals. Here, the crystal quality of Toho-1 was improved by using surface modification to remove a sulfate ion involved in crystal packing. The surface-modified Toho-1 variant (R274N/R276N) was crystallized under similar conditions to those used for wild-type Toho-1. R274N/R276N did not form merohedrally twinned crystals. The crystals diffracted to a significantly higher resolution (approximately 0.97 A) than the wild-type crystals (1.65 A); they belonged to the same space group and had almost identical unit-cell parameters to those of wild-type Toho-1.
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===Apo structure of class a beta-lactamase Toho-1 R274N/R276N double mutant===
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Improvement of crystal quality by surface mutations of beta-lactamase Toho-1.,Shimamura T, Nitanai Y, Uchiyama T, Matsuzawa H Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Apr 1;65(Pt, 4):379-82. Epub 2009 Mar 25. PMID:19342785<ref>PMID:19342785</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2zq8" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19342785}}, adds the Publication Abstract to the page
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*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19342785 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19342785}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2zq8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZQ8 OCA].
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==Reference==
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<ref group="xtra">PMID:019342785</ref><references group="xtra"/>
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[[Category: Beta-lactamase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Ago, H.]]
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[[Category: Large Structures]]
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[[Category: Matsuzawa, H.]]
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[[Category: Ago H]]
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[[Category: Miyano, M.]]
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[[Category: Matsuzawa H]]
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[[Category: Nitanai, Y.]]
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[[Category: Miyano M]]
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[[Category: Shimamura, T.]]
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[[Category: Nitanai Y]]
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[[Category: Uchiyama, T.]]
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[[Category: Shimamura T]]
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[[Category: Antibiotic resistance]]
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[[Category: Uchiyama T]]
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[[Category: Beta-lactamase]]
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[[Category: Esbl]]
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[[Category: Extended-spectrum]]
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[[Category: Hydrolase]]
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[[Category: Plasmid]]
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[[Category: Toho-1]]
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Current revision

Apo structure of class a beta-lactamase Toho-1 R274N/R276N double mutant

PDB ID 2zq8

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