2bky

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:17, 9 May 2024) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2bky.png|left|200px]]
 
-
<!--
+
==Crystal structure of the Alba1:Alba2 heterodimer from sulfolobus solfataricus==
-
The line below this paragraph, containing "STRUCTURE_2bky", creates the "Structure Box" on the page.
+
<StructureSection load='2bky' size='340' side='right'caption='[[2bky]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2bky]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BKY FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-
{{STRUCTURE_2bky| PDB=2bky | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bky OCA], [https://pdbe.org/2bky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bky RCSB], [https://www.ebi.ac.uk/pdbsum/2bky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bky ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ALBA1_SACS2 ALBA1_SACS2] Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. May be involved in DNA compaction. May bind rRNA and mRNA, playing a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/2bky_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bky ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Organisms growing at elevated temperatures face a particular challenge to maintain the integrity of their genetic material. All thermophilic and hyperthermophilic archaea encode one or more copies of the Alba (Sac10b) gene. Alba is an abundant, dimeric, highly basic protein that binds cooperatively and at high density to DNA. Sulfolobus solfataricus encodes a second copy of the Alba gene, and the Alba2 protein is expressed at approximately 5% of the level of Alba1. We demonstrate by NMR, ITC, and crystallography that Alba2 exists exclusively as a heterodimer with Alba1 at physiological concentrations and that heterodimerization exerts a clear effect upon the DNA packaging, as observed by EM, potentially by changing the interface between adjacent Alba dimers in DNA complexes. A functional role for Alba2 in modulation of higher order chromatin structure and DNA condensation is suggested.
-
===CRYSTAL STRUCTURE OF THE ALBA1:ALBA2 HETERODIMER FROM SULFOLOBUS SOLFATARICUS===
+
Obligate heterodimerization of the archaeal Alba2 protein with Alba1 provides a mechanism for control of DNA packaging.,Jelinska C, Conroy MJ, Craven CJ, Hounslow AM, Bullough PA, Waltho JP, Taylor GL, White MF Structure. 2005 Jul;13(7):963-71. PMID:16004869<ref>PMID:16004869</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_16004869}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2bky" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 16004869 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_16004869}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
[[2bky]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKY OCA].
+
[[Category: Saccharolobus solfataricus P2]]
-
 
+
[[Category: Bullough PA]]
-
==Reference==
+
[[Category: Conroy MJ]]
-
<ref group="xtra">PMID:016004869</ref><references group="xtra"/>
+
[[Category: Craven CJ]]
-
[[Category: Sulfolobus solfataricus]]
+
[[Category: Jelinska C]]
-
[[Category: Bullough, P A.]]
+
[[Category: Taylor GL]]
-
[[Category: Conroy, M J.]]
+
[[Category: Waltho JP]]
-
[[Category: Craven, C J.]]
+
[[Category: White MF]]
-
[[Category: Jelinska, C.]]
+
-
[[Category: Taylor, G L.]]
+
-
[[Category: Waltho, J P.]]
+
-
[[Category: White, M F.]]
+

Current revision

Crystal structure of the Alba1:Alba2 heterodimer from sulfolobus solfataricus

PDB ID 2bky

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bky"
Personal tools