2yjy
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 2yjy is ON HOLD until Paper Publication Authors: Dong, S., Wang, Y., Cassidy-Amstutz, C., Lu, G., Qiu, C., Bigler, R., Jezyk, M., Li, C., Hall, T.M....) |
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| - | '''Unreleased structure''' | ||
| - | + | ==A specific and modular binding code for cytosine recognition in PUF domains== | |
| + | <StructureSection load='2yjy' size='340' side='right'caption='[[2yjy]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2yjy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YJY FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.598Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yjy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjy OCA], [https://pdbe.org/2yjy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yjy RCSB], [https://www.ebi.ac.uk/pdbsum/2yjy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yjy ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PUM1_HUMAN PUM1_HUMAN] Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. May be required to support proliferation and self-renewal of stem cells (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Pumilio/fem-3 mRNA-binding factor (PUF) proteins possess a recognition code for bases A, U, and G, allowing designed RNA sequence specificity of their modular Pumilio (PUM) repeats. However, recognition side chains in a PUM repeat for cytosine are unknown. Here we report identification of a cytosine-recognition code by screening random amino acid combinations at conserved RNA recognition positions using a yeast three-hybrid system. This C-recognition code is specific and modular as specificity can be transferred to different positions in the RNA recognition sequence. A crystal structure of a modified PUF domain reveals specific contacts between an arginine side chain and the cytosine base. We applied the C-recognition code to design PUF domains that recognize targets with multiple cytosines and to generate engineered splicing factors that modulate alternative splicing. Finally, we identified a divergent yeast PUF protein, Nop9p, that may recognize natural target RNAs with cytosine. This work deepens our understanding of natural PUF protein target recognition and expands the ability to engineer PUF domains to recognize any RNA sequence. | ||
| - | + | Specific and Modular Binding Code for Cytosine Recognition in Pumilio/FBF (PUF) RNA-binding Domains.,Dong S, Wang Y, Cassidy-Amstutz C, Lu G, Bigler R, Jezyk MR, Li C, Hall TM, Wang Z J Biol Chem. 2011 Jul 29;286(30):26732-42. Epub 2011 Jun 8. PMID:21653694<ref>PMID:21653694</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 2yjy" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Bigler R]] | ||
| + | [[Category: Cassidy-Amstutz C]] | ||
| + | [[Category: Dong S]] | ||
| + | [[Category: Hall TMT]] | ||
| + | [[Category: Jezyk M]] | ||
| + | [[Category: Li C]] | ||
| + | [[Category: Lu G]] | ||
| + | [[Category: Qiu C]] | ||
| + | [[Category: Wang Y]] | ||
| + | [[Category: Wang Z]] | ||
Current revision
A specific and modular binding code for cytosine recognition in PUF domains
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Categories: Homo sapiens | Large Structures | Synthetic construct | Bigler R | Cassidy-Amstutz C | Dong S | Hall TMT | Jezyk M | Li C | Lu G | Qiu C | Wang Y | Wang Z
