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3asm

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[[Image:3asm.jpg|left|200px]]
 
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==Crystal structure of Q54A mutant protein of Bst-RNase HIII==
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The line below this paragraph, containing "STRUCTURE_3asm", creates the "Structure Box" on the page.
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<StructureSection load='3asm' size='340' side='right'caption='[[3asm]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3asm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ASM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ASM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.603&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3asm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3asm OCA], [https://pdbe.org/3asm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3asm RCSB], [https://www.ebi.ac.uk/pdbsum/3asm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3asm ProSAT]</span></td></tr>
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{{STRUCTURE_3asm| PDB=3asm | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6L6Q4_GEOSE Q6L6Q4_GEOSE] Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.[HAMAP-Rule:MF_00053][SAAS:SAAS00088233]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ribonuclease H3 from Bacillus stearothermophilus (Bst-RNase H3) has the N-terminal TBP-like substrate-binding domain. To identify the substrate binding site in this domain, the mutant proteins of the intact protein and isolated N-domain, in which six of the seventeen residues corresponding to those involved in DNA binding of TBP are individually mutated to Ala, were constructed. All of them exhibited decreased enzymatic activities and/or substrate-binding affinities when compared to those of the parent proteins, suggesting that the N-terminal domain of RNase H3 uses the flat surface of the beta-sheet for substrate binding as TBP to bind DNA. This domain may greatly change conformation upon substrate binding.
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===Crystal structure of Q54A mutant protein of Bst-RNase HIII===
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Identification of the substrate binding site in the N-terminal TBP-like domain of RNase H3.,Miyashita S, Tadokoro T, Angkawidjaja C, You DJ, Koga Y, Takano K, Kanaya S FEBS Lett. 2011 Jul 21;585(14):2313-7. Epub 2011 Jun 12. PMID:21664908<ref>PMID:21664908</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3asm" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3asm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ASM OCA].
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
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[[Category: Ribonuclease H]]
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[[Category: Large Structures]]
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[[Category: Angkawidjaja, C.]]
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[[Category: Angkawidjaja C]]
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[[Category: Kanaya, S.]]
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[[Category: Kanaya S]]

Current revision

Crystal structure of Q54A mutant protein of Bst-RNase HIII

PDB ID 3asm

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