3r1x
From Proteopedia
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| - | [[Image:3r1x.png|left|200px]] | ||
| - | < | + | ==Crystal structure of 2-oxo-3-deoxygalactonate kinase from Klebsiella pneumoniae== |
| - | + | <StructureSection load='3r1x' size='340' side='right'caption='[[3r1x]], [[Resolution|resolution]] 2.09Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3r1x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Klep7 Klep7]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3nuw 3nuw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R1X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R1X FirstGlance]. <br> | |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |
| - | -- | + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dgoK, KPN78578_40560, KPN_04097 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272620 KLEP7])</td></tr> | |
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/2-dehydro-3-deoxygalactonokinase 2-dehydro-3-deoxygalactonokinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.58 2.7.1.58] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r1x OCA], [https://pdbe.org/3r1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r1x RCSB], [https://www.ebi.ac.uk/pdbsum/3r1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r1x ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In most organisms, efficient D-galactose utilization requires the highly conserved Leloir pathway that converts D-galactose to D-glucose 1-phosphate. However, in some bacterial and fungal species alternative routes of D-galactose assimilation have been identified. In the so-called De Ley-Doudoroff pathway, D-galactose is metabolized into pyruvate and D-glyceraldehyde 3-phosphate in five consecutive reactions carried out by specific enzymes. The penultimate step in this pathway involves the phosphorylation of 2-oxo-3-deoxygalactonate to 2-oxo-3-deoxygalactonate 6-phosphate catalyzed by 2-oxo-3-deoxygalactonate kinase, with ATP serving as a phosphoryl-group donor. Here, a crystal structure of 2-oxo-3-deoxygalactonate kinase from Klebsiella pneumoniae determined at 2.1 A resolution is reported, the first structure of an enzyme from the De Ley-Doudoroff pathway. Structural comparison indicates that the enzyme belongs to the ASKHA (acetate and sugar kinases/hsc70/actin) family of phosphotransferases. The protein is composed of two alpha/beta domains, each of which contains a core common to all family members. Additional elements introduced between conserved structural motifs define the unique features of 2-oxo-3-deoxygalactonate kinase and possibly determine the biological function of the protein. | ||
| - | + | Structure of 2-oxo-3-deoxygalactonate kinase from Klebsiella pneumoniae.,Michalska K, Cuff ME, Tesar C, Feldmann B, Joachimiak A Acta Crystallogr D Biol Crystallogr. 2011 Aug;67(Pt 8):678-89. Epub 2011, Jul 12. PMID:21795809<ref>PMID:21795809</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 3r1x" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: 2-dehydro-3-deoxygalactonokinase]] | [[Category: 2-dehydro-3-deoxygalactonokinase]] | ||
| - | [[Category: | + | [[Category: Klep7]] |
| - | [[Category: Cuff, M E | + | [[Category: Large Structures]] |
| - | [[Category: Feldmann, B | + | [[Category: Cuff, M E]] |
| - | [[Category: Joachimiak, A | + | [[Category: Feldmann, B]] |
| - | [[Category: | + | [[Category: Joachimiak, A]] |
| - | [[Category: Michalska, K | + | [[Category: Structural genomic]] |
| - | [[Category: Tesar, C | + | [[Category: Michalska, K]] |
| + | [[Category: Tesar, C]] | ||
[[Category: 2-keto-3-deoxygalactonate kinase]] | [[Category: 2-keto-3-deoxygalactonate kinase]] | ||
[[Category: 2-oxo-3-deoxygalactonate kinase]] | [[Category: 2-oxo-3-deoxygalactonate kinase]] | ||
[[Category: Deley-doudoroff pathway]] | [[Category: Deley-doudoroff pathway]] | ||
[[Category: Mcsg]] | [[Category: Mcsg]] | ||
| - | [[Category: Midwest center for structural genomic]] | ||
[[Category: Psi-biology]] | [[Category: Psi-biology]] | ||
| - | [[Category: Structural genomic]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Current revision
Crystal structure of 2-oxo-3-deoxygalactonate kinase from Klebsiella pneumoniae
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