2xed

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[[Image:2xed.png|left|200px]]
 
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==Nocardia farcinica maleate cis-trans isomerase C194S mutant with a covalently bound succinylcysteine intermediate==
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The line below this paragraph, containing "STRUCTURE_2xed", creates the "Structure Box" on the page.
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<StructureSection load='2xed' size='340' side='right'caption='[[2xed]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xed]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Nocardia_farcinica_IFM_10152 Nocardia farcinica IFM 10152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XED OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XED FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr>
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{{STRUCTURE_2xed| PDB=2xed | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xed FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xed OCA], [https://pdbe.org/2xed PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xed RCSB], [https://www.ebi.ac.uk/pdbsum/2xed PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xed ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MAIA_NOCFA MAIA_NOCFA] Catalyzes cis-trans isomerization of the C2-C3 double bond in maleate to yield fumarate. Shows a strict specificity for maleate, with no activity detected toward structurally related substrates including citraconate, mesaconate, dimethylmaleate, and maleamide.<ref>PMID:20677745</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xe/2xed_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2xed ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Maleate isomerase (MI), a member of the Asp/Glu racemase superfamily, catalyzes cis-trans isomerization of the C2-C3 double bond in maleate to yield fumarate. Mutational studies, in conjunction with the structure of the C194A mutant of Nocardia farcinica MI cocrystallized with maleate, have revealed an unprecedented mode of catalysis for the superfamily in which the isomerization reaction is initiated by nucleophilic attack of cysteine at the double bond, yielding a covalent succinylcysteine-like intermediate.
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===NOCARDIA FARCINICA MALEATE CIS-TRANS ISOMERASE C194S MUTANT WITH A COVALENTLY BOUND SUCCINYLCYSTEINE INTERMEDIATE===
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A Covalent Succinylcysteine-like Intermediate in the Enzyme-Catalyzed Transformation of Maleate to Fumarate by Maleate Isomerase.,Fisch F, Fleites CM, Delenne M, Baudendistel N, Hauer B, Turkenburg JP, Hart S, Bruce NC, Grogan G J Am Chem Soc. 2010 Aug 2. PMID:20677745<ref>PMID:20677745</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20677745}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2xed" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20677745 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20677745}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[2xed]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Nocardia_farcinica Nocardia farcinica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XED OCA].
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[[Category: Nocardia farcinica IFM 10152]]
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[[Category: Baudendistel N]]
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==Reference==
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[[Category: Bruce NC]]
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<ref group="xtra">PMID:020677745</ref><references group="xtra"/>
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[[Category: Fisch F]]
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[[Category: Maleate isomerase]]
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[[Category: Grogan G]]
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[[Category: Nocardia farcinica]]
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[[Category: Hart S]]
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[[Category: Baudendistel, N.]]
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[[Category: Hauer B]]
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[[Category: Bruce, N C.]]
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[[Category: Martinez-Fleites C]]
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[[Category: Fisch, F.]]
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[[Category: Turkenburg JP]]
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[[Category: Grogan, G.]]
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[[Category: Hart, S.]]
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[[Category: Hauer, B.]]
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[[Category: Martinez-Fleites, C.]]
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[[Category: Turkenburg, J P.]]
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[[Category: Cofactor-independent cis-trans isomerase]]
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[[Category: Isomerase]]
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[[Category: Nicotinic acid catabolism]]
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Current revision

Nocardia farcinica maleate cis-trans isomerase C194S mutant with a covalently bound succinylcysteine intermediate

PDB ID 2xed

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