2y8w

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[[Image:2y8w.png|left|200px]]
 
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==Structure of CRISPR endoribonuclease Cse3 bound to 20 nt RNA==
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The line below this paragraph, containing "STRUCTURE_2y8w", creates the "Structure Box" on the page.
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<StructureSection load='2y8w' size='340' side='right'caption='[[2y8w]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2y8w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y8W FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8w OCA], [https://pdbe.org/2y8w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y8w RCSB], [https://www.ebi.ac.uk/pdbsum/2y8w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y8w ProSAT]</span></td></tr>
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{{STRUCTURE_2y8w| PDB=2y8w | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAS6_THET8 CAS6_THET8] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This enzyme processes pre-crRNA into individual crRNA units, but may not actually undergo enzyme turnover, retaining the crRNA product (PubMed:21572442). Generates a 2',3'-cyclic phosphodiester.<ref>PMID:21572442</ref> <ref>PMID:21572444</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Clustered regularly interspaced short palindromic repeat (CRISPR) chromosomal loci found in prokaryotes provide an adaptive immune system against bacteriophages and plasmids. CRISPR-specific endoRNases produce short RNA molecules (crRNAs) from CRISPR transcripts, which harbor sequences complementary to invasive nucleic acid elements and ensure their selective targeting by CRISPR-associated (Cas) proteins. The extreme sequence divergence of CRISPR-specific endoRNases and their RNA substrates has obscured homology-based comparison of RNA recognition and cleavage mechanisms. Here, we show that Cse3 type CRISPR-specific endoRNases bind a hairpin structure and residues downstream of the cleavage site within the repetitive segment of cognate CRISPR RNA. Cocrystal structures of Cse3-RNA complexes reveal an RNA-induced conformational change in the enzyme active site that aligns the RNA strand for site-specific cleavage. These studies provide insight into a catalytically essential RNA recognition mechanism by a large class of CRISPR-related endoRNases.
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===STRUCTURE OF CRISPR ENDORIBONUCLEASE CSE3 BOUND TO 20 NT RNA===
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An RNA-induced conformational change required for CRISPR RNA cleavage by the endoribonuclease Cse3.,Sashital DG, Jinek M, Doudna JA Nat Struct Mol Biol. 2011 Jun;18(6):680-7. Epub 2011 May 15. PMID:21572442<ref>PMID:21572442</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2y8w" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_21572442}}, adds the Publication Abstract to the page
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*[[CRISPR type I-E (Cascade)|CRISPR type I-E (Cascade)]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21572442 is the PubMed ID number.
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_21572442}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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[[2y8w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8W OCA].
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[[Category: Large Structures]]
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[[Category: Thermus thermophilus HB8]]
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==Reference==
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[[Category: Doudna JA]]
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<ref group="xtra">PMID:021572442</ref><references group="xtra"/>
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[[Category: Jinek M]]
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[[Category: Thermus thermophilus]]
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[[Category: Sashital DG]]
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[[Category: Doudna, J A.]]
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[[Category: Jinek, M.]]
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[[Category: Sashital, D G.]]
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[[Category: Ferredoxin-like]]
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[[Category: Hydrolase-rna complex]]
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Current revision

Structure of CRISPR endoribonuclease Cse3 bound to 20 nt RNA

PDB ID 2y8w

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