2yc1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:49, 20 December 2023) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2yc1.png|left|200px]]
 
-
<!--
+
==Crystal structure of the human derived single chain antibody fragment (scFv) 9004G in complex with Cn2 toxin from the scorpion Centruroides noxius Hoffmann==
-
The line below this paragraph, containing "STRUCTURE_2yc1", creates the "Structure Box" on the page.
+
<StructureSection load='2yc1' size='340' side='right'caption='[[2yc1]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2yc1]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Centruroides_noxius Centruroides noxius] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YC1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YC1 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-
{{STRUCTURE_2yc1| PDB=2yc1 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yc1 OCA], [https://pdbe.org/2yc1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yc1 RCSB], [https://www.ebi.ac.uk/pdbsum/2yc1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yc1 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SCX2_CENNO SCX2_CENNO] Mammal beta-toxins bind voltage-independently at site-4 of sodium channels and shift the activation voltage to more negative potentials. This toxin is active against mammals.
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
It has previously been reported that several single-chain antibody fragments of human origin (scFv) neutralize the effects of two different scorpion venoms through interactions with the primary toxins of Centruroides noxius Hoffmann (Cn2) and Centruroides suffusus suffusus (Css2). Here we present the crystal structure of the complex formed between one scFv (9004G) and the Cn2 toxin, determined in two crystal forms at 2.5 and 1.9 A resolution. A 15-residue span of the toxin is recognized by the antibody through a cleft formed by residues from five of the complementarity-determining regions of the scFv. Analysis of the interface of the complex reveals three features. First, the epitope of toxin Cn2 overlaps with essential residues for the binding of beta-toxins to its Na(+) channel receptor site. Second, the putative recognition of Css2 involves mainly residues that are present in both Cn2 and Css2 toxins. Finally, the effect on the increase of affinity of previously reported key residues during the maturation process of different scFvs can be inferred from the structure. Taken together, these results provide the structural basis that explain the mechanism of the 9004G neutralizing activity and give insight into the process of directed evolution that gave rise to this family of neutralizing scFvs.
-
===CRYSTAL STRUCTURE OF THE HUMAN DERIVED SINGLE CHAIN ANTIBODY FRAGMENT (SCFV) 9004G IN COMPLEX WITH CN2 TOXIN FROM THE SCORPION CENTRUROIDES NOXIUS HOFFMANN===
+
Structural Basis of Neutralization of the Major Toxic Component from the Scorpion Centruroides noxius Hoffmann by a Human-derived Single-chain Antibody Fragment.,Canul-Tec JC, Riano-Umbarila L, Rudino-Pinera E, Becerril B, Possani LD, Torres-Larios A J Biol Chem. 2011 Jun 10;286(23):20892-900. Epub 2011 Apr 13. PMID:21489992<ref>PMID:21489992</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2yc1" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_21489992}}, adds the Publication Abstract to the page
+
*[[Antibody 3D structures|Antibody 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 21489992 is the PubMed ID number.
+
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
-
-->
+
*[[3D structures of human antibody|3D structures of human antibody]]
-
{{ABSTRACT_PUBMED_21489992}}
+
== References ==
-
 
+
<references/>
-
==About this Structure==
+
__TOC__
-
[[2yc1]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Centruroides_noxius_hoffmann Centruroides noxius hoffmann] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YC1 OCA].
+
</StructureSection>
-
 
+
[[Category: Centruroides noxius]]
-
==Reference==
+
-
<ref group="xtra">PMID:021489992</ref><references group="xtra"/>
+
-
[[Category: Centruroides noxius hoffmann]]
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Becerril, B.]]
+
[[Category: Large Structures]]
-
[[Category: Canul-Tec, J C.]]
+
[[Category: Becerril B]]
-
[[Category: Possani, L D.]]
+
[[Category: Canul-Tec JC]]
-
[[Category: Riano-Umbarila, L.]]
+
[[Category: Possani LD]]
-
[[Category: Rudino-Pinera, E.]]
+
[[Category: Riano-Umbarila L]]
-
[[Category: Torres-Larios, A.]]
+
[[Category: Rudino-Pinera E]]
-
[[Category: Immune system-toxin complex]]
+
[[Category: Torres-Larios A]]
-
[[Category: Scorpion toxin]]
+

Current revision

Crystal structure of the human derived single chain antibody fragment (scFv) 9004G in complex with Cn2 toxin from the scorpion Centruroides noxius Hoffmann

PDB ID 2yc1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2yc1"
Personal tools