3mmj

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[[Image:3mmj.jpg|left|200px]]
 
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==Structure of the PTP-like phytase from Selenomonas ruminantium in complex with myo-inositol hexakisphosphate==
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The line below this paragraph, containing "STRUCTURE_3mmj", creates the "Structure Box" on the page.
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<StructureSection load='3mmj' size='340' side='right'caption='[[3mmj]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3mmj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Selenomonas_ruminantium Selenomonas ruminantium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MMJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MMJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_3mmj| PDB=3mmj | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mmj OCA], [https://pdbe.org/3mmj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mmj RCSB], [https://www.ebi.ac.uk/pdbsum/3mmj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mmj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7WUJ1_SELRU Q7WUJ1_SELRU]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein-tyrosine phosphatase-like inositol polyphosphatases are microbial enzymes that catalyze the stepwise removal of one or more phosphates from highly phosphorylated myo-inositols via a relatively ordered pathway. To understand the substrate specificity and kinetic mechanism of these enzymes we have determined high resolution, single crystal, x-ray crystallographic structures of inactive Selenomonas ruminantium PhyA in complex with myo-inositol hexa- and pentakisphosphate. These structures provide the first glimpse of a myo-inositol polyphosphatase-ligand complex consistent with its known specificity and reveal novel features of the kinetic mechanism. To complement the structural studies, fluorescent binding assays have been developed and demonstrate that the K(d) for this enzyme is several orders of magnitude lower than the K(m). Together with rapid kinetics data, these results suggest that the protein tyrosine phosphatase-like inositol polyphosphatases have a two-step, substrate-binding mechanism that facilitates catalysis.
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===Structure of the PTP-like phytase from Selenomonas ruminantium in complex with myo-inositol hexakisphosphate===
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Substrate binding in protein-tyrosine phosphatase-like inositol polyphosphatases.,Gruninger RJ, Dobing S, Smith AD, Bruder LM, Selinger LB, Wieden HJ, Mosimann SC J Biol Chem. 2012 Mar 23;287(13):9722-30. doi: 10.1074/jbc.M111.309872. Epub 2011, Dec 2. PMID:22139834<ref>PMID:22139834</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3mmj" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3mmj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Selenomonas_ruminantium Selenomonas ruminantium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MMJ OCA].
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*[[Phytase|Phytase]]
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*[[Phytase 3D structures|Phytase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Selenomonas ruminantium]]
[[Category: Selenomonas ruminantium]]
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[[Category: Gruninger, R J.]]
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[[Category: Gruninger RJ]]
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[[Category: Mosimann, S C.]]
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[[Category: Mosimann SC]]
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[[Category: Selinger, L B.]]
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[[Category: Selinger LB]]
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[[Category: Hydrolase]]
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[[Category: Inositol phosphatase]]
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[[Category: Inositol phosphate]]
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[[Category: Phytase]]
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[[Category: Protein tyrosine phosphatase]]
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Current revision

Structure of the PTP-like phytase from Selenomonas ruminantium in complex with myo-inositol hexakisphosphate

PDB ID 3mmj

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