2fbn

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(New page: 200px<br /><applet load="2fbn" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fbn, resolution 1.63&Aring;" /> '''Plasmodium falciparu...)
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[[Image:2fbn.gif|left|200px]]<br /><applet load="2fbn" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fbn, resolution 1.63&Aring;" />
 
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'''Plasmodium falciparum putative FK506-binding protein PFL2275c, C-terminal TPR-containing domain'''<br />
 
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==Overview==
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==Plasmodium falciparum putative FK506-binding protein PFL2275c, C-terminal TPR-containing domain==
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Parasites from the protozoan phylum Apicomplexa are responsible for, diseases, such as malaria, toxoplasmosis and cryptosporidiosis, all of, which have significantly higher rates of mortality and morbidity in, economically underdeveloped regions of the world. Advances in vaccine, development and drug discovery are urgently needed to control these, diseases and can be facilitated by production of purified recombinant, proteins from Apicomplexan genomes and determination of their 3D, structures. To date, both heterologous expression and crystallization of, Apicomplexan proteins have seen only limited success. In an effort to, explore the effectiveness of producing and crystallizing proteins on a, genome-scale using a standardized methodology, over 400 distinct, Plasmodium falciparum target genes were chosen representing different, cellular classes, along with select orthologues from four other Plasmodium, species as well as Cryptosporidium parvum and Toxoplasma gondii. From a, total of 1008 genes from the seven genomes, 304 (30.2%) produced purified, soluble proteins and 97 (9.6%) crystallized, culminating in 36 crystal, structures. These results demonstrate that, contrary to previous findings, a standardized platform using Escherichia coli can be effective for, genome-scale production and crystallography of Apicomplexan proteins., Predictably, orthologous proteins from different Apicomplexan genomes, behaved differently in expression, purification and crystallization, although the overall success rates of Plasmodium orthologues do not differ, significantly. Their differences were effectively exploited to elevate the, overall productivity to levels comparable to the most successful ongoing, structural genomics projects: 229 of the 468 target genes produced, purified soluble protein from one or more organisms, with 80 and 32 of the, purified targets, respectively, leading to crystals and ultimately, structures from one or more orthologues.
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<StructureSection load='2fbn' size='340' side='right'caption='[[2fbn]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2fbn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FBN FirstGlance]. <br>
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2FBN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FBN OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fbn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fbn OCA], [https://pdbe.org/2fbn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fbn RCSB], [https://www.ebi.ac.uk/pdbsum/2fbn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fbn ProSAT]</span></td></tr>
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==Reference==
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</table>
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Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms., Vedadi M, Lew J, Artz J, Amani M, Zhao Y, Dong A, Wasney GA, Gao M, Hills T, Brokx S, Qiu W, Sharma S, Diassiti A, Alam Z, Melone M, Mulichak A, Wernimont A, Bray J, Loppnau P, Plotnikova O, Newberry K, Sundararajan E, Houston S, Walker J, Tempel W, Bochkarev A, Kozieradzki I, Edwards A, Arrowsmith C, Roos D, Kain K, Hui R, Mol Biochem Parasitol. 2007 Jan;151(1):100-10. Epub 2006 Nov 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17125854 17125854]
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== Function ==
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[https://www.uniprot.org/uniprot/FKB35_PLAF7 FKB35_PLAF7] Has peptidylprolyl isomerase (PPIase) and co-chaperone activities (PubMed:15664653, PubMed:15850699). Assists protein folding by catalyzing the peptidyl conversion of cis and trans rotamers of the prolyl amide bond of protein substrates (PubMed:15664653, PubMed:15850699, PubMed:23974147). Inhibits calcineurin phosphatase activity in vitro (PubMed:15850699, PubMed:17289400, PubMed:23974147). Plays an essential role in merozoite egress from host erythrocytes (PubMed:15664653, PubMed:23974147).<ref>PMID:15664653</ref> <ref>PMID:15850699</ref> <ref>PMID:17289400</ref> <ref>PMID:23974147</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fb/2fbn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fbn ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Plasmodium falciparum]]
[[Category: Plasmodium falciparum]]
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[[Category: Single protein]]
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[[Category: Arrowsmith CH]]
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[[Category: Arrowsmith, C.H.]]
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[[Category: Bochkarev A]]
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[[Category: Bochkarev, A.]]
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[[Category: Dong A]]
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[[Category: Dong, A.]]
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[[Category: Edwards AM]]
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[[Category: Edwards, A.M.]]
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[[Category: Hills T]]
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[[Category: Hills, T.]]
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[[Category: Hui R]]
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[[Category: Hui, R.]]
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[[Category: Koeieradzki I]]
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[[Category: Koeieradzki, I.]]
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[[Category: Lew J]]
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[[Category: Lew, J.]]
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[[Category: Melone M]]
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[[Category: Melone, M.]]
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[[Category: Sundararajan E]]
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[[Category: SGC, Structural.Genomics.Consortium.]]
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[[Category: Sundstrom M]]
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[[Category: Sundararajan, E.]]
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[[Category: Wasney G]]
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[[Category: Sundstrom, M.]]
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[[Category: Weigelt J]]
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[[Category: Wasney, G.]]
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[[Category: Zhao Y]]
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[[Category: Weigelt, J.]]
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[[Category: Zhao, Y.]]
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[[Category: pfl2275c]]
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[[Category: sgc]]
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[[Category: structural genomics]]
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[[Category: structural genomics consortium]]
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[[Category: sulfur sad]]
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[[Category: tpr-containing domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:32:42 2008''
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Current revision

Plasmodium falciparum putative FK506-binding protein PFL2275c, C-terminal TPR-containing domain

PDB ID 2fbn

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