Beta-lactamase

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[[Image:3bm6.png|left|200px|thumb|Crystal Structure of Beta-lactamase, [[3bm6]]]]
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<StructureSection load='' size='350' side='right' scene='45/453531/Cv/1' caption='Class B β-lactamase Ndm-1 complex with Zn+2 (grey) (PDB code [[3spu]])'>
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{{STRUCTURE_3bm6| PDB=3bm6 | SIZE=300| SCENE=Beta-lactamase/Beta-lactamase/1|right|CAPTION=Beta-lactamase class D complex with boronic acid derivative, [[3bm6]] }}
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__TOC__
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== Function ==
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'''Beta-lactamase''' (Blac) are enzymes which make bacteria resitant to β-lactam antibiotics by breaking their β-lactam ring. Blac are classified according to their resistance targets. Class C are cephalosporinases; Class A are broad-spectrum; Class B are metalloenzymes; Class D are cloxacilanases.
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'''Beta-lactamase''' (Blac) are enzymes which make bacteria resistant to β-lactam antibiotics by breaking their β-lactam ring. Blac are classified according to their resistance targets.<br />
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*'''Class A''' are broad-spectrum. See also [[TEM1 Class Antibiotic Resistance Proteins]].<br />
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*'''Class B''' are metalloenzymes. See also [[New Delhi metallo-β-lactamase 1]].<br />
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*'''Class C''' are cephalosporinases. See also [[Class C beta-lactamase]].<br />
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{{TOC limit|limit=2}}
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*'''Class D''' are cloxacilanases<br />
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*'''Carbapenemase''' (Car) are Blac with wide hydrolytic capacities. Car can hydrolyze penicillins, cephalosporins, monobactams and carbapenems. <br />
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*'''Extended-spectrum Blac''' class A (ESBL) hydrolyze penicillins but not cephalosporins.<br />
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*'''Serine beta-lactamase''' (ClbP) has a serine-active site.
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*'''AmpC beta-lactamase''' (ClbP) mediate resistance to cephalosphin, cefazolin,cefoxitin and most penicillins<ref>PMID:19136439</ref>.
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== Relevance ==
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Blac makes some bacteria resistant to antibiotics like penicillin, cephamycin and carbapenems.
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== Structural highlights ==
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The <scene name='45/453531/Cv/3'>active site of class B Blac contains 2 Zn+2 ions</scene>.<ref>PMID:21774017</ref>
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==See also==
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[[Journal:Structure:1]]
== 3D Structures of Beta-lactamase ==
== 3D Structures of Beta-lactamase ==
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[[Beta-lactamase 3D structures]]
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===β-lactamase class A===
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</StructureSection>
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[[3dtm]] - EcBlac A residues 24-286 (mutant) - ''Escherichia coli''<br />
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[[3c7u]], [[3c7v]] – EcBlac A (mutant) + Protein Blip<br />
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[[1axb]] - EcBlac A (mutant) + transition state analog<br />
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[[1tem]] - EcBlac A (mutant) + penicillanic acid derivative<br />
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[[1o7e]] – Blac – ''Stenotrophonas maltophilia''
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===β-lactamase class B===
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[[3fcz]], [[3i0v]], [[3i11]], [[3i13]], [[3i14]], [[3i15]] – BcBlac B - ''Bacillus cereus''<br />
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[[3knr]], [[3kns]] - BcBlac C (mutant)
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===β-lactamase class C===
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[[3fkw]], [[3iwi]], [[3iwo]], [[3iwq]], [[3ixd]] – EcBlac C (mutant) <br />
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[[3bm6]], [[3fkv]], [[3o86]], [[3o87]], [[3o88]] – EcBlac C + boronic acid derivative<br />
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[[3ixb]], [[3ixg]] - EcBlac C (mutant) + boronic acid derivative<br />
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[[3ixh]] - EcBlac C (mutant) + cefotaxime<br />
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[[3gqz]], [[3gr2]], [[3grj]], [[3gsg]], [[3gtc]], [[3gv9]], [[3gvb]] - EcBlac C + inhibitor
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===β-lactamase class D===
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[[2rl3]], [[1k55]], [[1k57]], [[1k4e]], [[1ewz]], [[1fof]] – PaBlac D – ''Pseudomonas aeruginosa''<br />
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[[2hp5]], [[2hp6]], [[2hp9]], [[2hpb]] - PaBlac D (mutant) <br />
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[[1k6r]] - PaBlac D + moxalactam<br />
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[[1k6s]] - PaBlac D + phenylboronic acid<br />
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[[1k54]] - PaBlac D + penicillanic acid derivative<br />
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[[1bsg]] – Blac – ''Streptomyces albus''
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== References ==
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<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Class B β-lactamase Ndm-1 complex with Zn+2 (grey) (PDB code 3spu)

Drag the structure with the mouse to rotate

References

  1. ↑ Jacoby GA. AmpC beta-lactamases. Clin Microbiol Rev. 2009 Jan;22(1):161-82, Table of Contents. PMID:19136439 doi:10.1128/CMR.00036-08
  2. ↑ King D, Strynadka N. Crystal structure of New Delhi metallo-beta-lactamase reveals molecular basis for antibiotic resistance. Protein Sci. 2011 Sep;20(9):1484-91. doi: 10.1002/pro.697. Epub 2011 Aug, 2. PMID:21774017 doi:10.1002/pro.697

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Michal Harel, Jaime Prilusky, Alexander Berchansky, Joel L. Sussman

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