2fio

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(New page: 200px<br /><applet load="2fio" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fio, resolution 2.700&Aring;" /> '''Phage phi29 transcr...)
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[[Image:2fio.gif|left|200px]]<br /><applet load="2fio" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fio, resolution 2.700&Aring;" />
 
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'''Phage phi29 transcription regulator p4-DNA complex'''<br />
 
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==Overview==
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==Phage phi29 transcription regulator p4-DNA complex==
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Protein p4 affects the transcriptional switch that divides bacteriophage, phi29 infection in early and late phases. The synthesis of DNA replication, proteins and p4 takes place in the early phase, while structural, morphogenesis, and lysis proteins are synthesized in the late phase., Transcriptional switch by p4 is achieved by activating the late promoter, A3 and repressing the early promoters A2b and A2c. The crystal structure, of p4 alone and in complex with a 41 bp DNA, including the A3 promoter, binding site, helps us to understand how the phage cycle is controlled., Protein p4 has a unique alpha/beta fold that includes a DNA recognition, motif consisting of two N-terminal beta turn substructures, or N-hooks, located at the tips of an elongated protein homodimer. The two N-hooks, enter the major groove of the double helix, establishing base-specific, contacts. A high DNA curvature allows p4 N-hooks to reach two major groove, areas three helical turns apart, like a bow and its string.
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<StructureSection load='2fio' size='340' side='right'caption='[[2fio]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2fio]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_virus_phi29 Bacillus virus phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FIO FirstGlance]. <br>
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2FIO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_phage_f237 Vibrio phage f237]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FIO OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fio OCA], [https://pdbe.org/2fio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fio RCSB], [https://www.ebi.ac.uk/pdbsum/2fio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fio ProSAT]</span></td></tr>
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==Reference==
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</table>
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The structure of phage phi29 transcription regulator p4-DNA complex reveals an N-hook motif for DNA., Badia D, Camacho A, Perez-Lago L, Escandon C, Salas M, Coll M, Mol Cell. 2006 Apr 7;22(1):73-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16600871 16600871]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/TF4_BPPH2 TF4_BPPH2] Mediates, together with protein p6, the early to late transcriptional switch by stabilizing the binding of host RNA polymerase (RNAP) to the late A3 promoter. Activates transcription from the late A3 promoter and represses the main early promoters A2b and A2c by modifying the topology of the sequences encompassing early promoters A2c and A2b and late promoter A3 in a hairpin. Proteins p6 and p4 bind cooperatively to an approximately 200 bp DNA region located between the late A3 and the early A2c promoters. Binding of p4 molecules induces the reorganization of the binding of protein p6, giving rise to the nucleoprotein complex responsible for the switch from early to late transcription.<ref>PMID:11689446</ref> <ref>PMID:12426390</ref> <ref>PMID:14757050</ref> <ref>PMID:2107318</ref> <ref>PMID:21614197</ref> <ref>PMID:8617213</ref> <ref>PMID:9784366</ref>
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[[Category: Vibrio phage f237]]
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== References ==
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[[Category: Badia, D.]]
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<references/>
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[[Category: Camacho, A.]]
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__TOC__
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[[Category: Coll, M.]]
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</StructureSection>
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[[Category: Escandon, C.]]
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[[Category: Bacillus virus phi29]]
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[[Category: Perez-Lago, L.]]
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[[Category: Large Structures]]
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[[Category: Salas, M.]]
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[[Category: Badia D]]
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[[Category: protein-dna complex; n-hook dna-binding motif; transcription regulation]]
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[[Category: Camacho A]]
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[[Category: Coll M]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:37:19 2008''
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[[Category: Escandon C]]
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[[Category: Perez-Lago L]]
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[[Category: Salas M]]

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Phage phi29 transcription regulator p4-DNA complex

PDB ID 2fio

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