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2fja

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(New page: 200px<br /><applet load="2fja" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fja, resolution 2.000&Aring;" /> '''adenosine 5'-phosph...)
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[[Image:2fja.gif|left|200px]]<br /><applet load="2fja" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fja, resolution 2.000&Aring;" />
 
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'''adenosine 5'-phosphosulfate reductase in complex with substrate'''<br />
 
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==Overview==
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==adenosine 5'-phosphosulfate reductase in complex with substrate==
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The iron-sulfur flavoenzyme adenosine-5'-phosphosulfate (APS) reductase, catalyzes a key reaction of the global sulfur cycle by reversibly, transforming APS to sulfite and AMP. The structures of the dissimilatory, enzyme from Archaeoglobus fulgidus in the reduced state (FAD(red)) and in, the sulfite adduct state (FAD-sulfite-AMP) have been recently elucidated, at 1.6 and 2.5 A resolution, respectively. Here we present new structural, features of the enzyme trapped in four different catalytically relevant, states that provide us with a detailed picture of its reaction cycle. In, the oxidized state (FAD(ox)), the isoalloxazine moiety of the FAD cofactor, exhibits a similarly bent conformation as observed in the structure of the, reduced enzyme. In the APS-bound state (FAD(ox)-APS), the substrate APS is, embedded into a 17 A long substrate channel in such a way that the, isoalloxazine ring is pushed toward the channel bottom, thereby producing, a compressed enzyme-substrate complex. A clamp formed by residues ArgA317, and LeuA278 to fix the adenine ring and the curved APS conformation appear, to be key factors to hold APS in a strained conformation. This energy-rich, state is relaxed during the attack of APS on the reduced FAD. A relaxed, FAD-sulfite adduct is observed in the structure of the FAD-sulfite state., Finally, a FAD-sulfite-AMP1 state with AMP within van der Waals distance, of the sulfite adduct could be characterized. This structure documents how, adjacent negative charges are stabilized by the protein matrix which is, crucial for forming APS from AMP and sulfite in the reverse reaction.
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<StructureSection load='2fja' size='340' side='right'caption='[[2fja]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2fja]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FJA FirstGlance]. <br>
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2FJA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus] with <scene name='pdbligand=FAD:'>FAD</scene>, <scene name='pdbligand=SF4:'>SF4</scene> and <scene name='pdbligand=ADX:'>ADX</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenylyl-sulfate_reductase Adenylyl-sulfate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.99.2 1.8.99.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FJA OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADX:ADENOSINE-5-PHOSPHOSULFATE'>ADX</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fja OCA], [https://pdbe.org/2fja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fja RCSB], [https://www.ebi.ac.uk/pdbsum/2fja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fja ProSAT]</span></td></tr>
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Reaction mechanism of the iron-sulfur flavoenzyme adenosine-5'-phosphosulfate reductase based on the structural characterization of different enzymatic states., Schiffer A, Fritz G, Kroneck PM, Ermler U, Biochemistry. 2006 Mar 7;45(9):2960-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16503650 16503650]
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</table>
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[[Category: Adenylyl-sulfate reductase]]
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== Function ==
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[https://www.uniprot.org/uniprot/O28603_ARCFU O28603_ARCFU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fj/2fja_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fja ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Ermler, U.]]
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[[Category: Ermler U]]
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[[Category: Fritz, G.]]
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[[Category: Fritz G]]
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[[Category: Kroneck, P.M.]]
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[[Category: Kroneck PM]]
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[[Category: Schiffer, A.]]
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[[Category: Schiffer A]]
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[[Category: ADX]]
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[[Category: FAD]]
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[[Category: SF4]]
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[[Category: adenylyl-phosphosulfate reductase]]
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[[Category: aps reductase]]
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[[Category: sulfur cycle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:37:45 2008''
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Current revision

adenosine 5'-phosphosulfate reductase in complex with substrate

PDB ID 2fja

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