2fjt

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(New page: 200px<br /><applet load="2fjt" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fjt, resolution 1.901&Aring;" /> '''Adenylyl cyclase cl...)
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[[Image:2fjt.gif|left|200px]]<br /><applet load="2fjt" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fjt, resolution 1.901&Aring;" />
 
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'''Adenylyl cyclase class iv from Yersinia pestis'''<br />
 
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==Overview==
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==Adenylyl cyclase class iv from Yersinia pestis==
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The crystal structure of the class IV adenylyl cyclase (AC) from Yersinia, pestis (Yp) is reported at 1.9 A resolution. The class IV AC fold is, distinct from the previously described folds for class II and class III, ACs. The dimeric AC-IV folds into an antiparallel eight-stranded barrel, whose connectivity has been seen in only three previous structures: yeast, RNA triphosphatase and two proteins of unknown function from Pyrococcus, furiosus and Vibrio parahaemolyticus. Eight highly conserved ionic, residues E10, E12, K14, R63, K76, K111, D126, and E136 lie in the barrel, core and form the likely binding sites for substrate and divalent cations., A phosphate ion is observed bound to R63, K76, K111, and R113 near the, center of the conserved cluster. Unlike the AC-II and AC-III active sites, that utilize two-Asp motifs for cation binding, the AC-IV active site is, relatively enriched in glutamate and features an ExE motif as its most, conserved element. Homologs of Y. pestis AC-IV, including human thiamine, triphosphatase, span the three kingdoms of life and delineate an ancient, family of phosphonucleotide processing enzymes.
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<StructureSection load='2fjt' size='340' side='right'caption='[[2fjt]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2fjt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis_KIM10+ Yersinia pestis KIM10+]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FJT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FJT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.901&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fjt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fjt OCA], [https://pdbe.org/2fjt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fjt RCSB], [https://www.ebi.ac.uk/pdbsum/2fjt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fjt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A5P8YEL9_YERPE A0A5P8YEL9_YERPE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fj/2fjt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fjt ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2FJT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Adenylate_cyclase Adenylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.1 4.6.1.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FJT OCA].
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*[[3D Adenylyl cyclase 3D structures|3D Adenylyl cyclase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of the class IV adenylyl cyclase reveals a novel fold., Gallagher DT, Smith NN, Kim SK, Heroux A, Robinson H, Reddy PT, J Mol Biol. 2006 Sep 8;362(1):114-22. Epub 2006 Aug 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16905149 16905149]
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[[Category: Large Structures]]
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[[Category: Adenylate cyclase]]
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[[Category: Yersinia pestis KIM10+]]
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[[Category: Single protein]]
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[[Category: Gallagher DT]]
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[[Category: Yersinia pestis]]
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[[Category: Heroux A]]
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[[Category: Gallagher, D.T.]]
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[[Category: Kim S-K]]
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[[Category: Heroux, A.]]
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[[Category: Reddy PT]]
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[[Category: Kim, S.K.]]
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[[Category: Robinson H]]
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[[Category: Reddy, P.T.]]
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[[Category: Smith NN]]
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[[Category: Robinson, H.]]
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[[Category: Smith, N.N.]]
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[[Category: SO4]]
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[[Category: cyclase; beta barrel; dimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:38:07 2008''
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Current revision

Adenylyl cyclase class iv from Yersinia pestis

PDB ID 2fjt

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