2fk5

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(New page: 200px<br /><applet load="2fk5" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fk5, resolution 1.90&Aring;" /> '''Crystal structure of...)
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[[Image:2fk5.jpg|left|200px]]<br /><applet load="2fk5" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fk5, resolution 1.90&Aring;" />
 
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'''Crystal structure of l-fuculose-1-phosphate aldolase from Thermus thermophilus HB8'''<br />
 
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==About this Structure==
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==Crystal structure of l-fuculose-1-phosphate aldolase from Thermus thermophilus HB8==
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2FK5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/L-fuculose-phosphate_aldolase L-fuculose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.17 4.1.2.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FK5 OCA].
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<StructureSection load='2fk5' size='340' side='right'caption='[[2fk5]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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[[Category: L-fuculose-phosphate aldolase]]
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== Structural highlights ==
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[[Category: Single protein]]
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<table><tr><td colspan='2'>[[2fk5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FK5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FK5 FirstGlance]. <br>
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[[Category: Thermus thermophilus]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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[[Category: Jeyakanthan, J.]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fk5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fk5 OCA], [https://pdbe.org/2fk5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fk5 RCSB], [https://www.ebi.ac.uk/pdbsum/2fk5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fk5 ProSAT], [https://www.topsan.org/Proteins/RSGI/2fk5 TOPSAN]</span></td></tr>
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[[Category: Shiro, Y.]]
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</table>
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[[Category: CL]]
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== Function ==
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[[Category: SO4]]
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[https://www.uniprot.org/uniprot/Q5SHB9_THET8 Q5SHB9_THET8]
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[[Category: class ii aldolase]]
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== Evolutionary Conservation ==
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[[Category: fuculose phosphate]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: metal binding]]
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Check<jmol>
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[[Category: national project on protein structural and functional analyses]]
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<jmolCheckbox>
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[[Category: nppsfa]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fk/2fk5_consurf.spt"</scriptWhenChecked>
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[[Category: riken structural genomics/proteomics initiative]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: rsgi]]
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fk5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Fuculose phosphate aldolase catalyzes the reversible cleavage of L-fuculose-1-phosphate to dihydroxyacetone phosphate and L-lactaldehyde. The protein from Thermus thermophilus HB8 is a biological tetramer with a subunit molecular weight of 21 591 Da. Purified FucA has been crystallized using sitting-drop vapour-diffusion and microbatch techniques at 293 K. The crystals belong to space group P4, with unit-cell parameters a = b = 100.94, c = 45.87 A. The presence of a dimer of the enzyme in the asymmetric unit was estimated to give a Matthews coefficient (VM) of 2.7 A3 Da(-1) and a solvent content of 54.2%(v/v). Three-wavelength diffraction MAD data were collected to 2.3 A from zinc-containing crystals. Native diffraction data to 1.9 A resolution have been collected using synchrotron radiation at SPring-8.
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:38:18 2008''
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Purification, crystallization and preliminary X-ray crystallographic study of the L-fuculose-1-phosphate aldolase (FucA) from Thermus thermophilus HB8.,Jeyakanthan J, Taka J, Kikuchi A, Kuroishi C, Yutani K, Shiro Y Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Dec 1;61(Pt, 12):1075-7. Epub 2005 Nov 24. PMID:16511238<ref>PMID:16511238</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2fk5" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Aldolase 3D structures|Aldolase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Jeyakanthan J]]
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[[Category: Shiro Y]]

Current revision

Crystal structure of l-fuculose-1-phosphate aldolase from Thermus thermophilus HB8

PDB ID 2fk5

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