2flh

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(New page: 200px<br /><applet load="2flh" size="350" color="white" frame="true" align="right" spinBox="true" caption="2flh, resolution 1.20&Aring;" /> '''Crystal structure of...)
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[[Image:2flh.gif|left|200px]]<br /><applet load="2flh" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2flh, resolution 1.20&Aring;" />
 
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'''Crystal structure of cytokinin-specific binding protein from mung bean in complex with cytokinin'''<br />
 
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==Overview==
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==Crystal structure of cytokinin-specific binding protein from mung bean in complex with cytokinin==
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The cytosolic fraction of Vigna radiata contains a 17-kD protein that, binds plant hormones from the cytokinin group, such as zeatin. Using, recombinant protein and isothermal titration calorimetry as well as, fluorescence measurements coupled with ligand displacement, we have, reexamined the K(d) values and show them to range from approximately, 10(-6) M (for 4PU30) to 10(-4) M (for zeatin) for 1:1 stoichiometry, complexes. In addition, we have crystallized this cytokinin-specific, binding protein (Vr CSBP) in complex with zeatin and refined the structure, to 1.2 A resolution. Structurally, Vr CSBP is similar to plant, pathogenesis-related class 10 (PR-10) proteins, despite low sequence, identity (&lt;20%). This unusual fold conservation reinforces the notion that, classic PR-10 proteins have evolved to bind small-molecule ligands. The, fold consists of an antiparallel beta-sheet wrapped around a C-terminal, alpha-helix, with two short alpha-helices closing a cavity formed within, the protein core. In each of the four independent CSBP molecules, there is, a zeatin ligand located deep in the cavity with conserved conformation and, protein-ligand interactions. In three cases, an additional zeatin molecule, is found in variable orientation but with excellent definition in electron, density, which plugs the entrance to the binding pocket, sealing the inner, molecule from contact with bulk solvent.
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<StructureSection load='2flh' size='340' side='right'caption='[[2flh]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2flh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vigna_radiata Vigna radiata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FLH FirstGlance]. <br>
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2FLH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vigna_radiata Vigna radiata] with <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=ZEA:'>ZEA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZEA:(2E)-2-METHYL-4-(9H-PURIN-6-YLAMINO)BUT-2-EN-1-OL'>ZEA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2flh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2flh OCA], [https://pdbe.org/2flh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2flh RCSB], [https://www.ebi.ac.uk/pdbsum/2flh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2flh ProSAT]</span></td></tr>
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Crystal structure of Vigna radiata cytokinin-specific binding protein in complex with zeatin., Pasternak O, Bujacz GD, Fujimoto Y, Hashimoto Y, Jelen F, Otlewski J, Sikorski MM, Jaskolski M, Plant Cell. 2006 Oct;18(10):2622-34. Epub 2006 Sep 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16998071 16998071]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/PHBP_VIGRR PHBP_VIGRR] Binds the cytokinin trans-zeatin in vitro (PubMed:16998071, PubMed:18453695). Binds gibberellin A3 (GA3) in vitro (PubMed:25004979).<ref>PMID:16998071</ref> <ref>PMID:18453695</ref> <ref>PMID:25004979</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/2flh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2flh ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Vigna radiata]]
[[Category: Vigna radiata]]
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[[Category: Pasternak, O.]]
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[[Category: Bujacz GD]]
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[[Category: NA]]
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[[Category: Jaskolski M]]
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[[Category: ZEA]]
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[[Category: Pasternak O]]
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[[Category: cytokinins]]
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[[Category: Sikorski MM]]
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[[Category: multiple-ligand binding]]
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[[Category: pathogenesis-related proteins]]
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[[Category: zeatin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:39:21 2008''
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Current revision

Crystal structure of cytokinin-specific binding protein from mung bean in complex with cytokinin

PDB ID 2flh

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