3aly

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[[Image:3aly.jpg|left|200px]]
 
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==Crystal Structure of RNase HI from Sulfolobus tokodaii with C-terminal deletion==
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The line below this paragraph, containing "STRUCTURE_3aly", creates the "Structure Box" on the page.
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<StructureSection load='3aly' size='340' side='right'caption='[[3aly]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3aly]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurisphaera_tokodaii_str._7 Sulfurisphaera tokodaii str. 7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ALY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aly FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aly OCA], [https://pdbe.org/3aly PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aly RCSB], [https://www.ebi.ac.uk/pdbsum/3aly PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aly ProSAT]</span></td></tr>
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{{STRUCTURE_3aly| PDB=3aly | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNH_SULTO RNH_SULTO] Nuclease that specifically degrades the RNA of RNA-DNA hybrids. Endonucleolytically removes RNA primers from the Okazaki fragments of lagging strand synthesis on its own. In the presence of Mn(2+) or Co(2+) can also cleave an RNA-RNA hybrid; the dsRNase activity is 10- 100-fold lower than RNase H activity. Complements the temperature-sensitive phenotype of an E.coli double rnhA/rnhB (RNase H) disruption mutant.<ref>PMID:15520465</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RNase HI from the hyperthermophile Sulfolobus tokodaii (Sto-RNase HI) is stabilized by its C-terminal residues. In this work, the stabilization effect of the Sto-RNase HI C-terminal residues was investigated in detail by thermodynamic measurements of the stability of variants lacking the disulfide bond (C58/145A), or the six C-terminal residues (DeltaC6) and by structural analysis of DeltaC6. The results showed that the C-terminal does not affect overall structure and stabilization is caused by local interactions of the C-terminal, suggesting that the C-terminal residues could be used as a "stabilization tag." The Sto-RNase HI C-terminal residues (-IGCIILT) were introduced as a tag on three proteins. Each chimeric protein was more stable than its wild-type protein. These results suggested the possibility of a simple stabilization technique using a stabilization tag such as Sto-RNase HI C-terminal residues.
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===Crystal Structure of RNase HI from Sulfolobus tokodaii with C-terminal deletion===
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Stabilization by fusion to the C-terminus of hyperthermophile Sulfolobus tokodaii RNase HI: a possibility of protein stabilization tag.,Takano K, Okamoto T, Okada J, Tanaka S, Angkawidjaja C, Koga Y, Kanaya S PLoS One. 2011 Jan 19;6(1):e16226. PMID:21283826<ref>PMID:21283826</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3aly" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21283826 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21283826}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3aly]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii Sulfolobus tokodaii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ALY OCA].
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[[Category: Sulfurisphaera tokodaii str. 7]]
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[[Category: Angkawidjaja C]]
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==Reference==
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[[Category: Kanaya S]]
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<ref group="xtra">PMID:021283826</ref><references group="xtra"/>
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[[Category: Takano K]]
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[[Category: Ribonuclease H]]
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[[Category: Sulfolobus tokodaii]]
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[[Category: Angkawidjaja, C.]]
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[[Category: Kanaya, S.]]
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[[Category: Takano, K.]]
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[[Category: C-terminal anchor deletion]]
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[[Category: Hydrolase]]
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[[Category: Thermostable rnase hi]]
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Current revision

Crystal Structure of RNase HI from Sulfolobus tokodaii with C-terminal deletion

PDB ID 3aly

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