2l07

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[[Image:2l07.jpg|left|200px]]
 
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==1H, 13C, and 15N chemical shifts and structure of brazzein-derived peptide CKR-PNG==
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The line below this paragraph, containing "STRUCTURE_2l07", creates the "Structure Box" on the page.
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<StructureSection load='2l07' size='340' side='right'caption='[[2l07]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2l07]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pentadiplandra_brazzeana Pentadiplandra brazzeana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L07 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L07 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l07 OCA], [https://pdbe.org/2l07 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l07 RCSB], [https://www.ebi.ac.uk/pdbsum/2l07 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l07 ProSAT]</span></td></tr>
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{{STRUCTURE_2l07| PDB=2l07 | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Brazzein, a 54 residue sweet-tasting protein, is thought to participate in a multipoint binding interaction with the sweet taste receptor. Proposed sites for interaction with the receptor include 2 surface loops and the disulfide bond that connects the N- and C-termini. However, the importance of each site is not well understood. To characterize the structural role of the termini in the sweetness of brazzein, the position of the disulfide bond connecting the N- and C-termini was shifted by substituting K3-C4-K5 with C3-K4-R5. The apparent affinity and V(max) of the C3-K4-R5-brazzein (CKR-brazzein) variant were only modestly decreased compared with the wild-type (WT) brazzein. We determined a high-resolution structure of CKR-brazzein by nuclear magnetic resonance spectroscopy (backbone root mean square deviation of 0.39 A). Comparing the structure of CKR-brazzein with that of WT-brazzein revealed that the terminal beta-strands of the variant display extended beta-structure and increased dynamics relative to WT-brazzein. These results support previous mutagenesis studies and further suggest that, whereas interactions involving the termini are necessary for full function of brazzein, the termini do not constitute the primary site of interaction between brazzein and the sweet taste receptor.
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===1H, 13C, and 15N chemical shifts and structure of brazzein-derived peptide CKR-PNG===
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Structural Role of the Terminal Disulfide Bond in the Sweetness of Brazzein.,Dittli SM, Rao H, Tonelli M, Quijada J, Markley JL, Max M, Assadi-Porter F Chem Senses. 2011 Jul 15. PMID:21765060<ref>PMID:21765060</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[2l07]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pentadiplandra_brazzeana Pentadiplandra brazzeana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L07 OCA].
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<div class="pdbe-citations 2l07" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pentadiplandra brazzeana]]
[[Category: Pentadiplandra brazzeana]]
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[[Category: Assadi-Porter, F M.]]
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[[Category: Assadi-Porter FM]]
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[[Category: Dittli, S M.]]
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[[Category: Dittli SM]]
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[[Category: Rao, H.]]
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[[Category: Rao H]]
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[[Category: Tonelli, M.]]
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[[Category: Tonelli M]]
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[[Category: Plant protein]]
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Current revision

1H, 13C, and 15N chemical shifts and structure of brazzein-derived peptide CKR-PNG

PDB ID 2l07

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