2fon

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(New page: 200px<br /><applet load="2fon" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fon, resolution 2.740&Aring;" /> '''X-ray crystal struc...)
Current revision (09:24, 14 February 2024) (edit) (undo)
 
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[[Image:2fon.gif|left|200px]]<br /><applet load="2fon" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fon, resolution 2.740&Aring;" />
 
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'''X-ray crystal structure of LeACX1, an acyl-CoA oxidase from Lycopersicon esculentum (tomato)'''<br />
 
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==Overview==
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==X-ray crystal structure of LeACX1, an acyl-CoA oxidase from Lycopersicon esculentum (tomato)==
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The flavoenzyme acyl-CoA oxidase (ACX) catalyzes the first committed step, in beta-oxidation and is required for the biosynthesis of jasmonic acid, a, signaling molecule involved in plant defense. Recently, a mutant in tomato, was identified that is deficient in jasmonic acid production and, compromised in its wound response. This results from a single point, mutation in acx1, which causes the conserved residue Thr138 to be, substituted by isoleucine. To understand the structural basis for this, mutation, the crystal structure of LeACX1 was determined to 2.74 Angstrom, resolution by molecular replacement. Unexpectedly, an unusual packing, arrangement was observed in which three monomers of LeACX1 are present in, the asymmetric unit. Although the tertiary structure of LeACX1 is, essentially similar to the previously determined structures of ACX, enzymes, the packing within the unit cells is distinctly different.
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<StructureSection load='2fon' size='340' side='right'caption='[[2fon]], [[Resolution|resolution]] 2.74&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2fon]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_lycopersicum Solanum lycopersicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FON FirstGlance]. <br>
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2FON is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Solanum_lycopersicum Solanum lycopersicum] with <scene name='pdbligand=FAD:'>FAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Acyl-CoA_oxidase Acyl-CoA oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.3.6 1.3.3.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FON OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.74&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fon OCA], [https://pdbe.org/2fon PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fon RCSB], [https://www.ebi.ac.uk/pdbsum/2fon PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fon ProSAT]</span></td></tr>
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Structure determination and analysis of acyl-CoA oxidase (ACX1) from tomato., Powers RA, Rife CL, Schilmiller AL, Howe GA, Garavito RM, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):683-6. Epub 2006, May 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16699197 16699197]
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</table>
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[[Category: Acyl-CoA oxidase]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q5D8D3_SOLLC Q5D8D3_SOLLC]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fo/2fon_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fon ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Solanum lycopersicum]]
[[Category: Solanum lycopersicum]]
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[[Category: Garavito, R.M.]]
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[[Category: Garavito RM]]
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[[Category: Powers, R.A.]]
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[[Category: Powers RA]]
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[[Category: FAD]]
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[[Category: fad cofactor]]
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[[Category: oxidoreductase]]
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[[Category: peroxisomal beta-oxidation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:41:24 2008''
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Current revision

X-ray crystal structure of LeACX1, an acyl-CoA oxidase from Lycopersicon esculentum (tomato)

PDB ID 2fon

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