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3r4d

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[[Image:3r4d.jpg|left|200px]]
 
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==Crystal structure of mouse coronavirus receptor-binding domain complexed with its murine receptor==
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The line below this paragraph, containing "STRUCTURE_3r4d", creates the "Structure Box" on the page.
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<StructureSection load='3r4d' size='340' side='right'caption='[[3r4d]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3r4d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [https://en.wikipedia.org/wiki/Murine_coronavirus Murine coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R4D FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2ajf|2ajf]], [[3kbh|3kbh]], [[1l6z|1l6z]]</div></td></tr>
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{{STRUCTURE_3r4d| PDB=3r4d | SCENE= }}
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ceacam1, CEACAM1a-2S ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r4d OCA], [https://pdbe.org/3r4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r4d RCSB], [https://www.ebi.ac.uk/pdbsum/3r4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r4d ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/S5ZBM1_9BETC S5ZBM1_9BETC]] Spike protein S1: attaches the virion to the cell membrane by interacting with host receptor, initiating the infection.[HAMAP-Rule:MF_04099] Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099] Spike protein S2: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Coronaviruses have evolved diverse mechanisms to recognize different receptors for their cross-species transmission and host-range expansion. Mouse hepatitis coronavirus (MHV) uses the N-terminal domain (NTD) of its spike protein as its receptor-binding domain. Here we present the crystal structure of MHV NTD complexed with its receptor murine carcinoembryonic antigen-related cell adhesion molecule 1a (mCEACAM1a). Unexpectedly, MHV NTD contains a core structure that has the same beta-sandwich fold as human galectins (S-lectins) and additional structural motifs that bind to the N-terminal Ig-like domain of mCEACAM1a. Despite its galectin fold, MHV NTD does not bind sugars, but instead binds mCEACAM1a through exclusive protein-protein interactions. Critical contacts at the interface have been confirmed by mutagenesis, providing a structural basis for viral and host specificities of coronavirus/CEACAM1 interactions. Sugar-binding assays reveal that galectin-like NTDs of some coronaviruses such as human coronavirus OC43 and bovine coronavirus bind sugars. Structural analysis and mutagenesis localize the sugar-binding site in coronavirus NTDs to be above the beta-sandwich core. We propose that coronavirus NTDs originated from a host galectin and retained sugar-binding functions in some contemporary coronaviruses, but evolved new structural features in MHV for mCEACAM1a binding.
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===Structure of a novel galectin-like domain of mouse coronavirus complexed with its protein receptor===
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Crystal structure of mouse coronavirus receptor-binding domain complexed with its murine receptor.,Peng G, Sun D, Rajashankar KR, Qian Z, Holmes KV, Li F Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10696-701. Epub 2011 Jun 13. PMID:21670291<ref>PMID:21670291</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[3r4d]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_hepatitis_virus Murine hepatitis virus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4D OCA].
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<div class="pdbe-citations 3r4d" style="background-color:#fffaf0;"></div>
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[[Category: Murine hepatitis virus]]
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== References ==
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[[Category: Mus musculus]]
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<references/>
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[[Category: Holmes, K V.]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Lk3 transgenic mice]]
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[[Category: Murine coronavirus]]
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[[Category: Holmes, K V]]
[[Category: Li, F]]
[[Category: Li, F]]
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[[Category: Peng, G Q.]]
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[[Category: Peng, G Q]]
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[[Category: Qian, Z H.]]
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[[Category: Qian, Z H]]
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[[Category: Rajashankar, K R.]]
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[[Category: Rajashankar, K R]]
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[[Category: Sun, D W.]]
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[[Category: Sun, D W]]
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[[Category: 55]]
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[[Category: 70 mhv spike ntd - 192]]
[[Category: 70 mhv spike ntd - 192]]
[[Category: Beta-sandwich]]
[[Category: Beta-sandwich]]

Current revision

Crystal structure of mouse coronavirus receptor-binding domain complexed with its murine receptor

PDB ID 3r4d

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