3rj0

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[[Image:3rj0.jpg|left|200px]]
 
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==Plant steroid receptor BRI1 ectodomain in complex with brassinolide==
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The line below this paragraph, containing "STRUCTURE_3rj0", creates the "Structure Box" on the page.
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<StructureSection load='3rj0' size='340' side='right'caption='[[3rj0]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3rj0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RJ0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.541&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLD:BRASSINOLIDE'>BLD</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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{{STRUCTURE_3rj0| PDB=3rj0 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rj0 OCA], [https://pdbe.org/3rj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rj0 RCSB], [https://www.ebi.ac.uk/pdbsum/3rj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rj0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BRI1_ARATH BRI1_ARATH] Receptor with a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Regulates, in response to brassinosteroid binding, a signaling cascade involved in plant development, including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering. Binds brassinolide, and less effectively castasterone, but not 2,3,22,23-O-tetramethylbrassinolide or ecdysone. May be involved in a feedback regulation of brassinosteroid biosynthesis. Phosphorylates BRI1-associated receptor kinase 1 (BAK1), Transthyretin-Like protein (TTL) and SERK1 on 'Ser-299' and 'Thr-462' in vitro. May have a guanylyl cyclase activity.<ref>PMID:10557222</ref> <ref>PMID:10938344</ref> <ref>PMID:17138891</ref> <ref>PMID:17520012</ref> <ref>PMID:18694562</ref> <ref>PMID:19124768</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Polyhydroxylated steroids are regulators of body shape and size in higher organisms. In metazoans, intracellular receptors recognize these molecules. Plants, however, perceive steroids at membranes, using the membrane-integral receptor kinase BRASSINOSTEROID INSENSITIVE 1 (BRI1). Here we report the structure of the Arabidopsis thaliana BRI1 ligand-binding domain, determined by X-ray diffraction at 2.5 A resolution. We find a superhelix of 25 twisted leucine-rich repeats (LRRs), an architecture that is strikingly different from the assembly of LRRs in animal Toll-like receptors. A 70-amino-acid island domain between LRRs 21 and 22 folds back into the interior of the superhelix to create a surface pocket for binding the plant hormone brassinolide. Known loss- and gain-of-function mutations map closely to the hormone-binding site. We propose that steroid binding to BRI1 generates a docking platform for a co-receptor that is required for receptor activation. Our findings provide insight into the activation mechanism of this highly expanded family of plant receptors that have essential roles in hormone, developmental and innate immunity signalling.
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===Plant steroid receptor BRI1 ectodomain in complex with brassinolide===
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Structural basis of steroid hormone perception by the receptor kinase BRI1.,Hothorn M, Belkhadir Y, Dreux M, Dabi T, Noel JP, Wilson IA, Chory J Nature. 2011 Jun 12;474(7352):467-71. doi: 10.1038/nature10153. PMID:21666665<ref>PMID:21666665</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[3rj0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RJ0 OCA].
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<div class="pdbe-citations 3rj0" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
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[[Category: Hothorn, M.]]
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[[Category: Large Structures]]
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[[Category: Brassinosteroid binding]]
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[[Category: Hothorn M]]
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[[Category: Hormone receptor]]
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[[Category: Island domain]]
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[[Category: Leucine-rich repeat]]
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[[Category: N-glycosylation]]
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[[Category: Plasma membrane and endosome]]
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[[Category: Receptor kinase]]
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[[Category: Signaling protein]]
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[[Category: Steroid receptor]]
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[[Category: Superhelix]]
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Current revision

Plant steroid receptor BRI1 ectodomain in complex with brassinolide

PDB ID 3rj0

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