2fuk

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(New page: 200px<br /><applet load="2fuk" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fuk, resolution 1.6&Aring;" /> '''Crystal structure of ...)
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[[Image:2fuk.gif|left|200px]]<br /><applet load="2fuk" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fuk, resolution 1.6&Aring;" />
 
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'''Crystal structure of XC6422 from Xanthomonas campestris: a member of a/b serine hydrolase without lid at 1.6 resolution'''<br />
 
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==Overview==
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==Crystal structure of XC6422 from Xanthomonas campestris: a member of a/b serine hydrolase without lid at 1.6 resolution==
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XC6422 is a conserved hypothetical protein from Xanthomonas campestris, pathovar campestris (Xcc), a Gram-negative yellow-pigmented pathogenic, bacterium that causes black rot, one of the major worldwide diseases of, cruciferous crops. The protein consists of 220 amino acids and its, structure has been determined to 1.6 A resolution using the, multi-wavelength anomalous dispersion (MAD) method. Although it has very, low sequence identity to protein sequences in the PDB (less than 20%), the, determined structure nevertheless shows that it belongs to the superfamily, of serine alpha/beta-hydrolases, with an active site that is fully, accessible to solvent owing to the absence of a lid domain. Modelling, studies with the serine esterase inhibitor E600 indicate that XC6422, adopts a conserved Ser-His-Asp catalytic triad common to this superfamily, and has a preformed oxyanion hole for catalytic activation. These, structural features suggest that XC6422 is most likely to be a hydrolase, active on a soluble ester or a small lipid. An extra strand preceding the, first beta-strand in the canonical alpha/beta-hydrolase fold leads to, extensive subunit interactions between XC6422 monomers, which may explain, why XC6422 crystals of good diffraction quality can grow to dimensions of, up to 1.5 mm in a few days.
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<StructureSection load='2fuk' size='340' side='right'caption='[[2fuk]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2fuk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FUK FirstGlance]. <br>
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2FUK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Xanthomonas_campestris Xanthomonas campestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FUK OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fuk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fuk OCA], [https://pdbe.org/2fuk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fuk RCSB], [https://www.ebi.ac.uk/pdbsum/2fuk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fuk ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structure of XC6422 from Xanthomonas campestris at 1.6 A resolution: a small serine alpha/beta-hydrolase., Yang CY, Chin KH, Chou CC, Wang AH, Chou SH, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2006 Jun 1;62(Pt, 6):498-503. Epub 2006 May 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16754966 16754966]
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== Function ==
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[[Category: Protein complex]]
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[https://www.uniprot.org/uniprot/B0RM26_XANCB B0RM26_XANCB]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fu/2fuk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fuk ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Xanthomonas campestris]]
[[Category: Xanthomonas campestris]]
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[[Category: Chin, K.H.]]
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[[Category: Chin KH]]
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[[Category: Chou, C.C.]]
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[[Category: Chou CC]]
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[[Category: Chou, S.H.]]
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[[Category: Chou SH]]
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[[Category: Wang, A.H.J.]]
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[[Category: Wang AHJ]]
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[[Category: Yang, C.Y.]]
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[[Category: Yang CY]]
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[[Category: a/b hydrolase]]
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[[Category: structural genomics]]
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[[Category: x-ray diffraction]]
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[[Category: xanthomonas campestris]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:44:53 2008''
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Current revision

Crystal structure of XC6422 from Xanthomonas campestris: a member of a/b serine hydrolase without lid at 1.6 resolution

PDB ID 2fuk

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