2fwv

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(New page: 200px<br /><applet load="2fwv" size="350" color="white" frame="true" align="right" spinBox="true" caption="2fwv, resolution 1.700&Aring;" /> '''Crystal Structure o...)
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[[Image:2fwv.gif|left|200px]]<br /><applet load="2fwv" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fwv, resolution 1.700&Aring;" />
 
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'''Crystal Structure of Rv0813'''<br />
 
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==Overview==
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==Crystal Structure of Rv0813==
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The gene Rv0813c from Mycobacterium tuberculosis, which codes for a, hypothetical protein of unknown function, is conserved within the order, Actinomycetales but absent elsewhere. The crystal structure of Rv0813c, reveals a new family of proteins that resemble the fatty acid-binding, proteins (FABPs) found in eukaryotes. Rv0813c adopts the 10-stranded, beta-barrel fold typical of FABPs but lacks the double-helix insert that, covers the entry to the binding site in the eukaryotic proteins. The, barrel encloses a deep cavity, at the bottom of which a small cyclic, ligand was found to bind to the hydroxyl group of Tyr192. This residue is, part of a conserved Arg-X-Tyr motif much like the triad that binds the, carboxylate group of fatty acids in FABPs. Most of the residues forming, the internal surface of the cavity are conserved in homologous protein, sequences found in CG-rich prokaryotes, strongly suggesting that Rv0813c, is a member of a new family of bacterial FABP-like proteins that may have, roles in the recognition, transport, and/or storage of small molecules in, the bacterial cytosol.
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<StructureSection load='2fwv' size='340' side='right'caption='[[2fwv]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2fwv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FWV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FWV FirstGlance]. <br>
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2FWV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=DIO:'>DIO</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FWV OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fwv OCA], [https://pdbe.org/2fwv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fwv RCSB], [https://www.ebi.ac.uk/pdbsum/2fwv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fwv ProSAT]</span></td></tr>
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The crystal structure of Rv0813c from Mycobacterium tuberculosis reveals a new family of fatty acid-binding protein-like proteins in bacteria., Shepard W, Haouz A, Grana M, Buschiazzo A, Betton JM, Cole ST, Alzari PM, J Bacteriol. 2007 Mar;189(5):1899-904. Epub 2006 Dec 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17172346 17172346]
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</table>
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[[Category: Mycobacterium tuberculosis]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/NB2_MYCTU NB2_MYCTU] Heme-binding protein able to scavenge peroxynitrite and to protect free L-tyrosine against peroxynitrite-mediated nitration, by acting as a peroxynitrite isomerase that converts peroxynitrite to nitrate. Therefore, this protein likely plays a role in peroxynitrite sensing and in the detoxification of reactive nitrogen and oxygen species (RNS and ROS, respectively). Is able to bind nitric oxide (NO) in vitro, but may act as a sensor of peroxynitrite levels in vivo.[HAMAP-Rule:MF_01297]
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[[Category: Alzari, P.M.]]
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== Evolutionary Conservation ==
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[[Category: Betton, J.M.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Buschiazzo, A.]]
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Check<jmol>
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[[Category: Cole, S.T.]]
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<jmolCheckbox>
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[[Category: Grana, M.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fw/2fwv_consurf.spt"</scriptWhenChecked>
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[[Category: Haouz, A.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: SPINE, Structural.Proteomics.in.Europe.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Shepard, W.]]
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</jmolCheckbox>
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[[Category: DIO]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fwv ConSurf].
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[[Category: GOL]]
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<div style="clear:both"></div>
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[[Category: conserved hypothetical]]
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__TOC__
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[[Category: fatty acid binding protein like]]
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</StructureSection>
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[[Category: restricted to actinomycetes]]
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[[Category: Large Structures]]
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[[Category: spine]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: structural genomics]]
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[[Category: Alzari PM]]
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[[Category: structural proteomics in europe]]
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[[Category: Betton JM]]
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[[Category: unknown function]]
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[[Category: Buschiazzo A]]
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[[Category: Cole ST]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:46:34 2008''
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[[Category: Grana M]]
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[[Category: Haouz A]]
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[[Category: Shepard W]]

Current revision

Crystal Structure of Rv0813

PDB ID 2fwv

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