2g1g

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(New page: 200px<br /><applet load="2g1g" size="350" color="white" frame="true" align="right" spinBox="true" caption="2g1g" /> '''Solution structure of the anticodon loop of ...)
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[[Image:2g1g.gif|left|200px]]<br /><applet load="2g1g" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2g1g" />
 
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'''Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine'''<br />
 
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==Overview==
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==Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine==
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Modified nucleosides play an important role in structure and function of, tRNA. We have determined the solution structure of the anticodon stem-loop, (ASL) of initiator tRNA of Schizosaccharomyces pombe. The incorporation of, N6-threonylcarbamoyladenosine at the position 3' to the anticodon triplet, (t6A37) results in the formation of a U-turn motif and enhances stacking, interactions within the loop and stem regions (i.e. between A35 and t6A37), by bulging out U36. This conformation was not observed in a crystal, structure of tRNAi including the same modification in its anticodon loop, nor in the solution structure of the unmodified ASL. A t6A modification, also occurs in the well studied anti-stem-loop of lys-tRNA(UUU). A, comparison of this stem-loop with our structure demonstrates different, effects of the modification depending on the loop sequence.
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<StructureSection load='2g1g' size='340' side='right'caption='[[2g1g]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2g1g]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G1G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G1G FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=T6A:N-[N-(9-B-D-RIBOFURANOSYLPURIN-6-YL)CARBAMOYL]THREONINE-5-MONOPHOSPHATE'>T6A</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g1g OCA], [https://pdbe.org/2g1g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g1g RCSB], [https://www.ebi.ac.uk/pdbsum/2g1g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g1g ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Modified nucleosides play an important role in structure and function of tRNA. We have determined the solution structure of the anticodon stem-loop (ASL) of initiator tRNA of Schizosaccharomyces pombe. The incorporation of N6-threonylcarbamoyladenosine at the position 3' to the anticodon triplet (t6A37) results in the formation of a U-turn motif and enhances stacking interactions within the loop and stem regions (i.e. between A35 and t6A37) by bulging out U36. This conformation was not observed in a crystal structure of tRNAi including the same modification in its anticodon loop, nor in the solution structure of the unmodified ASL. A t6A modification also occurs in the well studied anti-stem-loop of lys-tRNA(UUU). A comparison of this stem-loop with our structure demonstrates different effects of the modification depending on the loop sequence.
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==About this Structure==
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The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif.,Lescrinier E, Nauwelaerts K, Zanier K, Poesen K, Sattler M, Herdewijn P Nucleic Acids Res. 2006 May 31;34(10):2878-86. Print 2006. PMID:16738127<ref>PMID:16738127</ref>
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2G1G is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G1G OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The naturally occurring N6-threonyl adenine in anticodon loop of Schizosaccharomyces pombe tRNAi causes formation of a unique U-turn motif., Lescrinier E, Nauwelaerts K, Zanier K, Poesen K, Sattler M, Herdewijn P, Nucleic Acids Res. 2006 May 31;34(10):2878-86. Print 2006. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16738127 16738127]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 2g1g" style="background-color:#fffaf0;"></div>
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[[Category: Lescrinier, E.]]
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== References ==
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[[Category: t-rnai anticodonloop t6a]]
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<references/>
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__TOC__
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 19:49:36 2008''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Lescrinier E]]

Current revision

Solution structure of the anticodon loop of S. Pombe tRNAi including the naturally occurring N6-threonyl adenine

PDB ID 2g1g

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