2wkk

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[[Image:2wkk.png|left|200px]]
 
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==Identification of the glycan target of the nematotoxic fungal galectin CGL2 in Caenorhabditis elegans==
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The line below this paragraph, containing "STRUCTURE_2wkk", creates the "Structure Box" on the page.
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<StructureSection load='2wkk' size='340' side='right'caption='[[2wkk]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wkk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WKK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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{{STRUCTURE_2wkk| PDB=2wkk | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wkk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wkk OCA], [https://pdbe.org/2wkk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wkk RCSB], [https://www.ebi.ac.uk/pdbsum/2wkk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wkk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CGL2_COPCI CGL2_COPCI] Binds lactose. May play a role in fruiting body formation.<ref>PMID:8999822</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wk/2wkk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wkk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The physiological role of fungal galectins has remained elusive. Here, we show that feeding of a mushroom galectin, Coprinopsis cinerea CGL2, to Caenorhabditis elegans inhibited development and reproduction and ultimately resulted in killing of this nematode. The lack of toxicity of a carbohydrate-binding defective CGL2 variant and the resistance of a C. elegans mutant defective in GDP-fucose biosynthesis suggested that CGL2-mediated nematotoxicity depends on the interaction between the galectin and a fucose-containing glycoconjugate. A screen for CGL2-resistant worm mutants identified this glycoconjugate as a Galbeta1,4Fucalpha1,6 modification of C. elegans N-glycan cores. Analysis of N-glycan structures in wild type and CGL2-resistant nematodes confirmed this finding and allowed the identification of a novel putative glycosyltransferase required for the biosynthesis of this glycoepitope. The X-ray crystal structure of a complex between CGL2 and the Galbeta1,4Fucalpha1,6GlcNAc trisaccharide at 1.5 A resolution revealed the biophysical basis for this interaction. Our results suggest that fungal galectins play a role in the defense of fungi against predators by binding to specific glycoconjugates of these organisms.
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===IDENTIFICATION OF THE GLYCAN TARGET OF THE NEMATOTOXIC FUNGAL GALECTIN CGL2 IN CAENORHABDITIS ELEGANS===
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Caenorhabditis elegans N-glycan core beta-galactoside confers sensitivity towards nematotoxic fungal galectin CGL2.,Butschi A, Titz A, Walti MA, Olieric V, Paschinger K, Nobauer K, Guo X, Seeberger PH, Wilson IB, Aebi M, Hengartner MO, Kunzler M PLoS Pathog. 2010 Jan;6(1):e1000717. Epub 2010 Jan 8. PMID:20062796<ref>PMID:20062796</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2wkk" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20062796}}, adds the Publication Abstract to the page
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*[[Galectin 3D structures|Galectin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20062796 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20062796}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2wkk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Coprinopsis_cinerea Coprinopsis cinerea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WKK OCA].
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==Reference==
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<ref group="xtra">PMID:020062796</ref><references group="xtra"/>
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[[Category: Coprinopsis cinerea]]
[[Category: Coprinopsis cinerea]]
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[[Category: Aebi, M.]]
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[[Category: Large Structures]]
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[[Category: Butschi, A.]]
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[[Category: Aebi M]]
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[[Category: Hengartner, M O.]]
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[[Category: Butschi A]]
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[[Category: Kuenzler, M.]]
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[[Category: Hengartner MO]]
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[[Category: Olieric, V.]]
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[[Category: Kuenzler M]]
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[[Category: Paschinger, K.]]
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[[Category: Olieric V]]
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[[Category: Seeberger, P H.]]
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[[Category: Paschinger K]]
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[[Category: Titz, A.]]
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[[Category: Seeberger PH]]
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[[Category: Waelti, M.]]
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[[Category: Titz A]]
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[[Category: Wilson, I B.H.]]
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[[Category: Waelti M]]
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[[Category: Xiaoqiang, G.]]
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[[Category: Wilson IBH]]
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[[Category: Beta-galactoside binding lectin]]
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[[Category: Xiaoqiang G]]
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[[Category: Cell wall]]
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[[Category: Extracellular matrix]]
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[[Category: Fruiting body]]
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[[Category: Galectin]]
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[[Category: Lectin]]
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[[Category: Secreted]]
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[[Category: Sugar binding]]
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[[Category: Sugar binding protein]]
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[[Category: Sugar-binding protein]]
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Current revision

Identification of the glycan target of the nematotoxic fungal galectin CGL2 in Caenorhabditis elegans

PDB ID 2wkk

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