3otx

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "3otx" [edit=sysop:move=sysop])
Current revision (16:57, 1 November 2023) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3otx.jpg|left|200px]]
 
-
<!--
+
==Crystal Structure of Trypanosoma brucei rhodesiense Adenosine Kinase Complexed with Inhibitor AP5A==
-
The line below this paragraph, containing "STRUCTURE_3otx", creates the "Structure Box" on the page.
+
<StructureSection load='3otx' size='340' side='right'caption='[[3otx]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3otx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OTX FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AP5:BIS(ADENOSINE)-5-PENTAPHOSPHATE'>AP5</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-
{{STRUCTURE_3otx| PDB=3otx | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3otx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3otx OCA], [https://pdbe.org/3otx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3otx RCSB], [https://www.ebi.ac.uk/pdbsum/3otx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3otx ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q584S0_TRYB2 Q584S0_TRYB2]
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
BACKGROUND: The essential purine salvage pathway of Trypanosoma brucei bears interesting catalytic enzymes for chemotherapeutic intervention of Human African Trypanosomiasis. Unlike mammalian cells, trypanosomes lack de novo purine synthesis and completely rely on salvage from their hosts. One of the key enzymes is adenosine kinase which catalyzes the phosphorylation of ingested adenosine to form adenosine monophosphate (AMP) utilizing adenosine triphosphate (ATP) as the preferred phosphoryl donor. METHODS AND FINDINGS: Here, we present the first structures of Trypanosoma brucei rhodesiense adenosine kinase (TbrAK): the structure of TbrAK in complex with the bisubstrate inhibitor P(1),P(5)-di(adenosine-5')-pentaphosphate (AP5A) at 1.55 A, and TbrAK complexed with the recently discovered activator 4-[5-(4-phenoxyphenyl)-2H-pyrazol-3-yl]morpholine (compound 1) at 2.8 A resolution. CONCLUSIONS: The structural details and their comparison give new insights into substrate and activator binding to TbrAK at the molecular level. Further structure-activity relationship analyses of a series of derivatives of compound 1 support the observed binding mode of the activator and provide a possible mechanism of action with respect to their activating effect towards TbrAK.
-
===Crystal Structure of Trypanosoma brucei rhodesiense Adenosine Kinase Complexed with Inhibitor AP5A===
+
Crystal Structures of T. b. rhodesiense Adenosine Kinase Complexed with Inhibitor and Activator: Implications for Catalysis and Hyperactivation.,Kuettel S, Greenwald J, Kostrewa D, Ahmed S, Scapozza L, Perozzo R PLoS Negl Trop Dis. 2011 May;5(5):e1164. Epub 2011 May 24. PMID:21629723<ref>PMID:21629723</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_21629723}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 3otx" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 21629723 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_21629723}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
[[3otx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTX OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:021629723</ref><references group="xtra"/>
+
-
[[Category: Adenosine kinase]]
+
[[Category: Trypanosoma brucei]]
[[Category: Trypanosoma brucei]]
-
[[Category: Ahmed, S.]]
+
[[Category: Ahmed S]]
-
[[Category: Greenwald, J.]]
+
[[Category: Greenwald J]]
-
[[Category: Kostrewa, D.]]
+
[[Category: Kostrewa D]]
-
[[Category: Kuettel, S.]]
+
[[Category: Kuettel S]]
-
[[Category: Perozzo, R.]]
+
[[Category: Perozzo R]]
-
[[Category: Scapozza, L.]]
+
[[Category: Scapozza L]]
-
[[Category: Adenosine kinase]]
+
-
[[Category: Ap5a]]
+
-
[[Category: Transferase-transferase inhibitor complex]]
+

Current revision

Crystal Structure of Trypanosoma brucei rhodesiense Adenosine Kinase Complexed with Inhibitor AP5A

PDB ID 3otx

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3otx"
Personal tools