2gj2

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(New page: 200px<br /><applet load="2gj2" size="350" color="white" frame="true" align="right" spinBox="true" caption="2gj2, resolution 2.35&Aring;" /> '''Crystal Structure of...)
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[[Image:2gj2.gif|left|200px]]<br /><applet load="2gj2" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gj2, resolution 2.35&Aring;" />
 
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'''Crystal Structure of VP9 from White Spot Syndrome Virus'''<br />
 
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==Overview==
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==Crystal Structure of VP9 from White Spot Syndrome Virus==
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White spot syndrome virus (WSSV) is a major pathogen in shrimp, aquaculture. VP9, a full-length protein of WSSV, encoded by open reading, frame wsv230, was identified for the first time in the infected Penaeus, monodon shrimp tissues, gill, and stomach as a novel, nonstructural, protein by Western blotting, mass spectrometry, and immunoelectron, microscopy. Real-time reverse transcription-PCR demonstrated that the, transcription of VP9 started from the early to the late stage of WSSV, infection as a major mRNA species. The structure of full-length VP9 was, determined by both X-ray and nuclear magnetic resonance (NMR) techniques., It is the first structure to be reported for WSSV proteins. The crystal, structure of VP9 revealed a ferredoxin fold with divalent metal ion, binding sites. Cadmium sulfate was found to be essential for, crystallization. The Cd2+ ions were bound between the monomer interfaces, of the homodimer. Various divalent metal ions have been titrated against, VP9, and their interactions were analyzed using NMR spectroscopy. The, titration data indicated that VP9 binds with both Zn2+ and Cd2+. VP9, adopts a similar fold as the DNA binding domain of the papillomavirus E2, protein. Based on our present investigations, we hypothesize that VP9, might be involved in the transcriptional regulation of WSSV, a function, similar to that of the E2 protein during papillomavirus infection of the, host cells.
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<StructureSection load='2gj2' size='340' side='right'caption='[[2gj2]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2gj2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shrimp_white_spot_syndrome_virus Shrimp white spot syndrome virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GJ2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gj2 OCA], [https://pdbe.org/2gj2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gj2 RCSB], [https://www.ebi.ac.uk/pdbsum/2gj2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gj2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q91LD0_9VIRU Q91LD0_9VIRU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gj/2gj2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gj2 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2GJ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Shrimp_white_spot_syndrome_virus Shrimp white spot syndrome virus] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GJ2 OCA].
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Identification of a novel nonstructural protein, VP9, from white spot syndrome virus: its structure reveals a ferredoxin fold with specific metal binding sites., Liu Y, Wu J, Song J, Sivaraman J, Hew CL, J Virol. 2006 Nov;80(21):10419-27. Epub 2006 Sep 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16956937 16956937]
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[[Category: Large Structures]]
[[Category: Shrimp white spot syndrome virus]]
[[Category: Shrimp white spot syndrome virus]]
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[[Category: Single protein]]
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[[Category: Hew CL]]
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[[Category: Hew, C.L.]]
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[[Category: Liu Y]]
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[[Category: Liu, Y.]]
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[[Category: Sivaraman J]]
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[[Category: Sivaraman, J.]]
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[[Category: Song JX]]
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[[Category: Song, J.X.]]
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[[Category: Wu JL]]
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[[Category: Wu, J.L.]]
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[[Category: CD]]
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[[Category: ferredoxin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:00:49 2008''
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Current revision

Crystal Structure of VP9 from White Spot Syndrome Virus

PDB ID 2gj2

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OCA

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