2gmd

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(New page: 200px<br /><applet load="2gmd" size="350" color="white" frame="true" align="right" spinBox="true" caption="2gmd" /> '''Structure of C12-LF11 bound to the SDS micel...)
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[[Image:2gmd.jpg|left|200px]]<br /><applet load="2gmd" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gmd" />
 
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'''Structure of C12-LF11 bound to the SDS micelles'''<br />
 
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==Overview==
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==Structure of C12-LF11 bound to the SDS micelles==
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Incorporation of an acyl chain into the peptides extends their range of activities. In known lipopeptide structures the peptide and non-peptidyl segment are segregated into two separate structural domains. Solution NMR conformation of the cationic antimicrobial lipopeptide C12LF11 (N-lauryl undecapeptide LF11 based on human lactoferrin) shows a completely reorganized peptide conformation in micellar environment in comparison to the parent peptide. As a striking difference to other known lipopeptide structures, the acyl chain of C12LF11 forms an integral part of the hydrophobic cluster with long-range effects on the peptide conformation. The peptide C-terminus forms two helical segments and is better defined in DPC than in SDS micelles. Peptide acylation improved particularly the activity against the bacterial strains with short oligosaccharide (rough) LPS chemotype and protected mice against the endotoxic shock. The profound structural effects of acylation demonstrate that this type of modification allows a manipulation of the peptide's conformation and improves their bioactivity.
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<StructureSection load='2gmd' size='340' side='right'caption='[[2gmd]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2gmd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GMD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GMD FirstGlance]. <br>
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2GMD is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NH2:'>NH2</scene> and <scene name='pdbligand=DAO:'>DAO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GMD OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gmd OCA], [https://pdbe.org/2gmd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gmd RCSB], [https://www.ebi.ac.uk/pdbsum/2gmd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gmd ProSAT]</span></td></tr>
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The acyl group as the central element of the structural organization of antimicrobial lipopeptide., Japelj B, Zorko M, Majerle A, Pristovsek P, Sanchez-Gomez S, Tejada GM, Moriyon I, Blondelle SE, Brandenburg K, Andra J, Lohner K, Jerala R, J Am Chem Soc. 2007 Feb 7;129(5):1022-3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17263370 17263370]
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</table>
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[[Category: Protein complex]]
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== Function ==
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[[Category: Japelj, B.]]
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[https://www.uniprot.org/uniprot/TRFL_HUMAN TRFL_HUMAN] Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.<ref>PMID:12535064</ref> <ref>PMID:22320386</ref> Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.<ref>PMID:12535064</ref> <ref>PMID:22320386</ref> Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.<ref>PMID:12535064</ref> <ref>PMID:22320386</ref> The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.<ref>PMID:12535064</ref> <ref>PMID:22320386</ref> Isoform DeltaLf: transcription factor with antiproliferative properties and inducing cell cycle arrest. Binds to DeltaLf response element found in the SKP1, BAX, DCPS, and SELH promoters.<ref>PMID:12535064</ref> <ref>PMID:22320386</ref>
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[[Category: DAO]]
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== References ==
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[[Category: NH2]]
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<references/>
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[[Category: peptide]]
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__TOC__
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</StructureSection>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:03:11 2008''
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[[Category: Large Structures]]
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[[Category: Synthetic construct]]
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[[Category: Japelj B]]

Current revision

Structure of C12-LF11 bound to the SDS micelles

PDB ID 2gmd

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