2xhl

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[[Image:2xhl.png|left|200px]]
 
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==Structure of a functional derivative of Clostridium botulinum neurotoxin type B==
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The line below this paragraph, containing "STRUCTURE_2xhl", creates the "Structure Box" on the page.
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<StructureSection load='2xhl' size='340' side='right'caption='[[2xhl]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xhl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XHL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2xhl| PDB=2xhl | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhl OCA], [https://pdbe.org/2xhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xhl RCSB], [https://www.ebi.ac.uk/pdbsum/2xhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xhl ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BXB_CLOBO BXB_CLOBO] Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-|-Phe-77' bond of synaptobrevin-2.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Botulinum neurotoxins (BoNTs) cause flaccid paralysis by inhibiting neurotransmission at cholinergic nerve terminals. BoNTs consist of three essential domains for toxicity: the cell binding domain (Hc), the translocation domain (Hn) and the catalytic domain (LC). A functional derivative (LHn) of the parent neurotoxin B composed of Hn and LC domains was recombinantly produced and characterised. LHn/B crystallographic structure at 2.8A resolution is reported. The catalytic activity of LHn/B towards recombinant human VAMP was analysed by substrate cleavage assay and showed a higher specificity for VAMP-1, -2 compared to VAMP-3. LHn/B also showed measurable activity in living spinal cord neurons. Despite lacking the Hc (cell-targeting) domain, LHn/B retained the capacity to internalize and cleave intracellular VAMP-1 and -2 when added to the cells at high concentration. These activities of the LHn/B fragment demonstrate the utility of engineered botulinum neurotoxin fragments as analytical tools to study the mechanisms of action of BoNT neurotoxins and of SNARE proteins.
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===STRUCTURE OF A FUNCTIONAL DERIVATIVE OF CLOSTRIDIUM BOTULINUM NEUROTOXIN TYPE B===
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Structure and activity of a functional derivative of Clostridium botulinum neurotoxin B.,Masuyer G, Beard M, Cadd VA, Chaddock JA, Acharya KR J Struct Biol. 2010 Nov 13. PMID:21078393<ref>PMID:21078393</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2xhl" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Botulinum neurotoxin 3D structures|Botulinum neurotoxin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21078393 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21078393}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2xhl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHL OCA].
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==Reference==
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<ref group="xtra">PMID:021078393</ref><references group="xtra"/>
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[[Category: Bontoxilysin]]
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[[Category: Clostridium botulinum]]
[[Category: Clostridium botulinum]]
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[[Category: Acharya, K R.]]
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[[Category: Large Structures]]
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[[Category: Beard, M.]]
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[[Category: Acharya KR]]
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[[Category: Cadd, V A.]]
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[[Category: Beard M]]
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[[Category: Chaddock, J A.]]
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[[Category: Cadd VA]]
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[[Category: Masuyer, G.]]
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[[Category: Chaddock JA]]
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[[Category: Botulism]]
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[[Category: Masuyer G]]
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[[Category: Endopeptidase]]
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[[Category: Hydrolase]]
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[[Category: Membrane domain]]
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[[Category: Metalloprotease]]
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[[Category: Toxin]]
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[[Category: Zinc protease]]
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Current revision

Structure of a functional derivative of Clostridium botulinum neurotoxin type B

PDB ID 2xhl

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