3hkx

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[[Image:3hkx.png|left|200px]]
 
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==Crystal structure analysis of an amidase from Nesterenkonia sp.==
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The line below this paragraph, containing "STRUCTURE_3hkx", creates the "Structure Box" on the page.
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<StructureSection load='3hkx' size='340' side='right'caption='[[3hkx]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3hkx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nesterenkonia_sp._10004 Nesterenkonia sp. 10004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HKX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HKX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hkx OCA], [https://pdbe.org/3hkx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hkx RCSB], [https://www.ebi.ac.uk/pdbsum/3hkx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hkx ProSAT]</span></td></tr>
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{{STRUCTURE_3hkx| PDB=3hkx | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/D0VWZ1_9MICC D0VWZ1_9MICC]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/3hkx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hkx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Nesterenkonia strain AN1 was isolated from a screening program for nitrile- and amide-hydrolyzing microorganisms in Antarctic desert soil samples. Strain AN1 showed significant 16S rRNA sequence identity to known members of the genus. Like known Nesterenkonia species, strain AN1 was obligately alkaliphilic (optimum environmental pH, 9 to 10) and halotolerant (optimum environmental Na(+) content, 0 to 15% [wt/vol]) but was also shown to be an obligate psychrophile with optimum growth at approximately 21 degrees C. The partially sequenced genome of AN1 revealed an open reading frame (ORF) encoding a putative protein member of the nitrilase superfamily, referred to as NitN (264 amino acids). The protein crystallized readily as a dimer and the atomic structure of all but 10 amino acids of the protein was determined, confirming that the enzyme had an active site and a fold characteristic of the nitrilase superfamily. The protein was screened for activity against a variety of nitrile, carbamoyl, and amide substrates and was found to have only amidase activity. It had highest affinity for propionamide but demonstrated a low catalytic rate. NitN had maximal activity at 30 degrees C and between pH 6.5 and 7.5, conditions which are outside the optimum growth range for the organism.
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===Crystal structure analysis of an amidase from Nesterenkonia sp.===
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Unique aliphatic amidase from a psychrotrophic and haloalkaliphilic nesterenkonia isolate.,Nel AJ, Tuffin IM, Sewell BT, Cowan DA Appl Environ Microbiol. 2011 Jun;77(11):3696-702. Epub 2011 Apr 15. PMID:21498772<ref>PMID:21498772</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_21498772}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3hkx" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21498772 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21498772}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3hkx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nesterenkonia_sp._10004 Nesterenkonia sp. 10004]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HKX OCA].
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==Reference==
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<ref group="xtra">PMID:021498772</ref><references group="xtra"/>
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[[Category: Amidase]]
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[[Category: Nesterenkonia sp. 10004]]
[[Category: Nesterenkonia sp. 10004]]
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[[Category: Cowan, D A.]]
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[[Category: Cowan DA]]
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[[Category: Nel, A J.M.]]
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[[Category: Nel AJM]]
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[[Category: Sewell, B T.]]
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[[Category: Sewell BT]]
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[[Category: Alpha-beta-beta-alpha:alpha-beta-beta-alpha dimeric sandwich]]
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[[Category: Hydrolase]]
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Current revision

Crystal structure analysis of an amidase from Nesterenkonia sp.

PDB ID 3hkx

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