2xcz

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[[Image:2xcz.png|left|200px]]
 
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==Crystal Structure of macrophage migration inhibitory factor homologue from Prochlorococcus marinus==
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The line below this paragraph, containing "STRUCTURE_2xcz", creates the "Structure Box" on the page.
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<StructureSection load='2xcz' size='340' side='right'caption='[[2xcz]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xcz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Prochlorococcus_marinus_str._MIT_9313 Prochlorococcus marinus str. MIT 9313]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XCZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XCZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.64&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
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{{STRUCTURE_2xcz| PDB=2xcz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xcz OCA], [https://pdbe.org/2xcz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xcz RCSB], [https://www.ebi.ac.uk/pdbsum/2xcz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xcz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7V867_PROMM Q7V867_PROMM]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xc/2xcz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2xcz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Macrophage migration inhibitory factor (MIF) is a multifunctional mammalian cytokine, which exhibits tautomerase and oxidoreductase activity. MIF homologues with pairwise sequence identities to human MIF ranging from 31% to 41% have been detected in various cyanobacteria. The gene encoding the MIF homologue from the marine cyanobacterium Prochlorococcus marinus strain MIT9313 has been cloned and the corresponding protein (PmMIF) overproduced, purified, and subjected to functional and structural characterization. Kinetic and (1)H NMR spectroscopic studies show that PmMIF tautomerizes phenylenolpyruvate and (p-hydroxyphenyl)enolpyruvate at low levels. The N-terminal proline of PmMIF is critical for these reactions because the P1A mutant has strongly reduced tautomerase activities. PmMIF shows high structural homology with mammalian MIFs as revealed by a crystal structure of PmMIF at 1.63 A resolution. MIF contains a Cys-X-X-Cys motif that mediates oxidoreductase activity, which is lacking from PmMIF. Engineering of the motif into PmMIF did not result in oxidoreductase activity but increased the tautomerase activity 8-fold. The shared tautomerase activities and the conservation of the beta-alpha-beta structural fold and key functional groups suggest that eukaryotic MIFs and cyanobacterial PmMIF are related by divergent evolution from a common ancestor. While several MIF homologues have been identified in eukaryotic parasites, where they are thought to play a role in modulating the host immune response, PmMIF is the first nonparasitic, bacterial MIF-like protein characterized in detail. This work sets the stage for future studies which could address the question whether a MIF-like protein from a free-living bacterium possesses immunostimulatory features similar to those of mammalian MIFs and MIF-like proteins found in parasitic nematodes and protozoa.
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===CRYSTAL STRUCTURE OF MACROPHAGE MIGRATION INHIBITORY FACTOR HOMOLOGUE FROM PROCHLOROCOCCUS MARINUS===
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Structural and Functional Characterization of a Macrophage Migration Inhibitory Factor Homologue from the Marine Cyanobacterium Prochlorococcus marinus .,Wasiel AA, Rozeboom HJ, Hauke D, Baas BJ, Zandvoort E, Quax WJ, Thunnissen AM, Poelarends GJ Biochemistry. 2010 Aug 17. PMID:20715791<ref>PMID:20715791</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20715791}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2xcz" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20715791 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20715791}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[2xcz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Prochlorococcus_marinus Prochlorococcus marinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XCZ OCA].
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[[Category: Prochlorococcus marinus str. MIT 9313]]
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[[Category: Baas BJ]]
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==Reference==
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[[Category: Hauke D]]
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<ref group="xtra">PMID:020715791</ref><references group="xtra"/>
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[[Category: Poelarends GJ]]
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[[Category: Prochlorococcus marinus]]
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[[Category: Quax WJ]]
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[[Category: Baas, B J.]]
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[[Category: Rozeboom HJ]]
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[[Category: Hauke, D.]]
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[[Category: Thunnissen AMWH]]
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[[Category: Poelarends, G J.]]
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[[Category: Wasiel AA]]
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[[Category: Quax, W J.]]
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[[Category: Zandvoort E]]
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[[Category: Rozeboom, H J.]]
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[[Category: Thunnissen, A M.W H.]]
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[[Category: Wasiel, A A.]]
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[[Category: Zandvoort, E.]]
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[[Category: Cyanobacterium]]
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[[Category: Cytokine]]
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[[Category: Immune system]]
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[[Category: Tautomerase]]
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Current revision

Crystal Structure of macrophage migration inhibitory factor homologue from Prochlorococcus marinus

PDB ID 2xcz

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