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2gq5

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(New page: 200px<br /><applet load="2gq5" size="350" color="white" frame="true" align="right" spinBox="true" caption="2gq5, resolution 1.4&Aring;" /> '''Crystal structure of ...)
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[[Image:2gq5.gif|left|200px]]<br /><applet load="2gq5" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gq5, resolution 1.4&Aring;" />
 
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'''Crystal structure of an RNA racemate'''<br />
 
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==Overview==
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==Crystal structure of an RNA racemate==
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The racemate of the RNA duplex r(CUGGGCGG).r(CCGCCUGG) from Thermus flavus, 5S rRNA has been crystallized and examined by X-ray crystallography. The, space group is P1(_) with approximate unit-cell parameters a = 26.5, b =, 38.0, c = 45.4 Angstrom, alpha = 113.1, beta = 100.5, gamma = 93.3, degrees. The structure was solved by molecular replacement. There are four, RNA duplexes in the unit cell. The crystal lattice consists of columns of, RNA duplexes. The duplexes are stacked end-to-end and are stabilized by, intermolecular base-stacking interactions. Within each column the, L-duplexes and D-duplexes are stacked alternately. Every other duplex in, each stack has two alternative conformations, approximately equally, occupied, corresponding to molecules oriented in opposite directions., Neighbouring columns are related by the crystallographic centre of, symmetry. The unit cell also contains approximately 250 ordered water, molecules and six ordered calcium ions. A glycerol molecule is visible in, the minor groove interacting with a guanosine residue.
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<StructureSection load='2gq5' size='340' side='right'caption='[[2gq5]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2gq5]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GQ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GQ5 FirstGlance]. <br>
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2GQ5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GQ5 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0C:L-CYTIDINE-5-MONOPHOSPHATE'>0C</scene>, <scene name='pdbligand=0G:L-GUANOSINE-5-MONOPHOSPHATE'>0G</scene>, <scene name='pdbligand=0U:L-URIDINE-5-MONOPHOSPHATE'>0U</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gq5 OCA], [https://pdbe.org/2gq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gq5 RCSB], [https://www.ebi.ac.uk/pdbsum/2gq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gq5 ProSAT]</span></td></tr>
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The first crystal structure of an RNA racemate., Rypniewski W, Vallazza M, Perbandt M, Klussmann S, Delucas LJ, Betzel C, Erdmann VA, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):659-64. Epub 2006, May 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16699193 16699193]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Betzel, C.]]
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</StructureSection>
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[[Category: Erdmann, V.A.]]
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[[Category: Large Structures]]
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[[Category: Klussmann, S.]]
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[[Category: Betzel C]]
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[[Category: Perbandt, M.]]
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[[Category: Erdmann VA]]
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[[Category: Rypniewski, W.]]
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[[Category: Klussmann S]]
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[[Category: Vallazza, M.]]
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[[Category: Perbandt M]]
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[[Category: CA]]
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[[Category: Rypniewski W]]
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[[Category: GOL]]
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[[Category: Vallazza M]]
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[[Category: double helix]]
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[[Category: racemate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:05:33 2008''
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Current revision

Crystal structure of an RNA racemate

PDB ID 2gq5

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