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2gxs

From Proteopedia

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(New page: 200px<br /><applet load="2gxs" size="350" color="white" frame="true" align="right" spinBox="true" caption="2gxs, resolution 1.66&Aring;" /> '''HERA N-terminal doma...)
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[[Image:2gxs.gif|left|200px]]<br /><applet load="2gxs" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gxs, resolution 1.66&Aring;" />
 
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'''HERA N-terminal domain in complex with AMP, crystal form 2'''<br />
 
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==Overview==
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==HERA N-terminal domain in complex with AMP, crystal form 2==
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DEAD box RNA helicases use the energy of ATP hydrolysis to unwind, double-stranded RNA regions or to disrupt RNA/protein complexes. A minimal, RNA helicase comprises nine conserved motifs distributed over two, RecA-like domains. The N-terminal domain contains all motifs involved in, nucleotide binding, namely the Q-motif, the DEAD box, and the P-loop, as, well as the SAT motif, which has been implicated in the coordination of, ATP hydrolysis and RNA unwinding. We present here the crystal structure of, the N-terminal domain of the Thermus thermophilus RNA helicase Hera in, complex with adenosine monophosphate (AMP). Upon binding of AMP the P-loop, adopts a partially collapsed or half-open conformation that is still, connected to the DEAD box motif, and the DEAD box in turn is linked to the, SAT motif via hydrogen bonds. This network of interactions communicates, changes in the P-loop conformation to distant parts of the helicase. The, affinity of AMP is comparable to that of ADP and ATP, substantiating that, the binding energy from additional phosphate moieties is directly, converted into conformational changes of the entire helicase. Importantly, the N-terminal Hera domain forms a dimer in the crystal similar to that, seen in another thermophilic prokaryote. It is possible that this mode of, dimerization represents the prototypic architecture in RNA helicases of, thermophilic origin.
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<StructureSection load='2gxs' size='340' side='right'caption='[[2gxs]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2gxs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GXS FirstGlance]. <br>
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2GXS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=AMP:'>AMP</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GXS OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gxs OCA], [https://pdbe.org/2gxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gxs RCSB], [https://www.ebi.ac.uk/pdbsum/2gxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gxs ProSAT]</span></td></tr>
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Crystal structure and nucleotide binding of the Thermus thermophilus RNA helicase Hera N-terminal domain., Rudolph MG, Heissmann R, Wittmann JG, Klostermeier D, J Mol Biol. 2006 Aug 25;361(4):731-43. Epub 2006 Jul 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16890241 16890241]
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</table>
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[[Category: Single protein]]
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== Function ==
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[[Category: Thermus thermophilus]]
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[https://www.uniprot.org/uniprot/O07897_THETH O07897_THETH]
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[[Category: Klostermeier, D.]]
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== Evolutionary Conservation ==
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[[Category: Rudolph, M.G.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: AMP]]
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Check<jmol>
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[[Category: CA]]
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<jmolCheckbox>
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[[Category: GOL]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gx/2gxs_consurf.spt"</scriptWhenChecked>
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[[Category: amp complex]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: atomic resolution]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: ribosome biogenesis]]
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</jmolCheckbox>
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[[Category: rna helicase]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gxs ConSurf].
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[[Category: thermophilic]]
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<div style="clear:both"></div>
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__TOC__
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:10:13 2008''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Thermus thermophilus HB27]]
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[[Category: Klostermeier D]]
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[[Category: Rudolph MG]]

Current revision

HERA N-terminal domain in complex with AMP, crystal form 2

PDB ID 2gxs

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