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| - | [[Image:3sag.jpg|left|200px]] | |
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| - | <!-- | + | ==Crystal structure of the human RRP6 catalytic domain with D313N mutation in the active site== |
| - | The line below this paragraph, containing "STRUCTURE_3sag", creates the "Structure Box" on the page.
| + | <StructureSection load='3sag' size='340' side='right'caption='[[3sag]], [[Resolution|resolution]] 2.70Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | + | <table><tr><td colspan='2'>[[3sag]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SAG FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | -->
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene></td></tr> |
| - | {{STRUCTURE_3sag| PDB=3sag | SCENE= }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sag OCA], [https://pdbe.org/3sag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sag RCSB], [https://www.ebi.ac.uk/pdbsum/3sag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sag ProSAT]</span></td></tr> |
| | + | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/EXOSX_HUMAN EXOSX_HUMAN] Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA.<ref>PMID:14527413</ref> <ref>PMID:16455498</ref> <ref>PMID:17412707</ref> <ref>PMID:17545563</ref> <ref>PMID:18172165</ref> <ref>PMID:19056938</ref> <ref>PMID:20699273</ref> <ref>PMID:20368444</ref> |
| | | | |
| - | ===Crystal structure of the human RRP6 catalytic domain with D313N mutation in the active site=== | + | ==See Also== |
| - | | + | *[[Exosome 3D structures|Exosome 3D structures]] |
| - | | + | == References == |
| - | ==About this Structure== | + | <references/> |
| - | [[3sag]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SAG OCA].
| + | __TOC__ |
| | + | </StructureSection> |
| | [[Category: Homo sapiens]] | | [[Category: Homo sapiens]] |
| - | [[Category: Januszyk, K.]] | + | [[Category: Large Structures]] |
| - | [[Category: Lima, C D.]] | + | [[Category: Januszyk K]] |
| - | [[Category: Liu, Q.]] | + | [[Category: Lima CD]] |
| - | [[Category: Exoribonuclease]] | + | [[Category: Liu Q]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Rna exosome]]
| + | |
| Structural highlights
Function
EXOSX_HUMAN Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA.[1] [2] [3] [4] [5] [6] [7] [8]
See Also
References
- ↑ Lejeune F, Li X, Maquat LE. Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities. Mol Cell. 2003 Sep;12(3):675-87. PMID:14527413
- ↑ West S, Gromak N, Norbury CJ, Proudfoot NJ. Adenylation and exosome-mediated degradation of cotranscriptionally cleaved pre-messenger RNA in human cells. Mol Cell. 2006 Feb 3;21(3):437-43. PMID:16455498 doi:http://dx.doi.org/10.1016/j.molcel.2005.12.008
- ↑ Schilders G, van Dijk E, Pruijn GJ. C1D and hMtr4p associate with the human exosome subunit PM/Scl-100 and are involved in pre-rRNA processing. Nucleic Acids Res. 2007;35(8):2564-72. Epub 2007 Apr 4. PMID:17412707 doi:http://dx.doi.org/10.1093/nar/gkm082
- ↑ van Dijk EL, Schilders G, Pruijn GJ. Human cell growth requires a functional cytoplasmic exosome, which is involved in various mRNA decay pathways. RNA. 2007 Jul;13(7):1027-35. Epub 2007 Jun 1. PMID:17545563 doi:10.1261/rna.575107
- ↑ Mullen TE, Marzluff WF. Degradation of histone mRNA requires oligouridylation followed by decapping and simultaneous degradation of the mRNA both 5' to 3' and 3' to 5'. Genes Dev. 2008 Jan 1;22(1):50-65. doi: 10.1101/gad.1622708. PMID:18172165 doi:10.1101/gad.1622708
- ↑ Preker P, Nielsen J, Kammler S, Lykke-Andersen S, Christensen MS, Mapendano CK, Schierup MH, Jensen TH. RNA exosome depletion reveals transcription upstream of active human promoters. Science. 2008 Dec 19;322(5909):1851-4. doi: 10.1126/science.1164096. Epub 2008, Dec 4. PMID:19056938 doi:10.1126/science.1164096
- ↑ de Almeida SF, Garcia-Sacristan A, Custodio N, Carmo-Fonseca M. A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination. Nucleic Acids Res. 2010 Dec;38(22):8015-26. doi: 10.1093/nar/gkq703. Epub 2010, Aug 10. PMID:20699273 doi:http://dx.doi.org/10.1093/nar/gkq703
- ↑ Slomovic S, Fremder E, Staals RH, Pruijn GJ, Schuster G. Addition of poly(A) and poly(A)-rich tails during RNA degradation in the cytoplasm of human cells. Proc Natl Acad Sci U S A. 2010 Apr 20;107(16):7407-12. doi:, 10.1073/pnas.0910621107. Epub 2010 Apr 5. PMID:20368444 doi:10.1073/pnas.0910621107
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