2hmt

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(New page: 200px<br /><applet load="2hmt" size="350" color="white" frame="true" align="right" spinBox="true" caption="2hmt, resolution 2.200&Aring;" /> '''Diamond-shaped octa...)
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[[Image:2hmt.jpg|left|200px]]<br /><applet load="2hmt" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hmt, resolution 2.200&Aring;" />
 
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'''Diamond-shaped octameric ring structure of an RCK domain with NADH bound'''<br />
 
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==Overview==
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==Diamond-shaped octameric ring structure of an RCK domain with NADH bound==
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The KtrAB ion transporter is a complex of the KtrB membrane protein and, KtrA, an RCK domain. RCK domains regulate eukaryotic and prokaryotic, membrane proteins involved in K(+) transport. Conflicting functional, models have proposed two different oligomeric arrangements for RCK, domains, tetramer versus octamer. Our results for the KtrAB RCK domain, clearly show an octamer in solution and in the crystal. We determined the, structure of this protein in three different octameric ring conformations, that resemble the RCK-domain octamer observed in the MthK potassium, channel but show striking differences in size and symmetry. We present, experimental evidence for the association between one RCK octameric ring, and two KtrB membrane proteins. These results provide insights into the, quaternary organization of the KtrAB transporter and its mechanism of, activation and show that the RCK-domain octameric ring model is generally, applicable to other ion-transport systems.
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<StructureSection load='2hmt' size='340' side='right'caption='[[2hmt]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hmt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HMT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hmt OCA], [https://pdbe.org/2hmt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hmt RCSB], [https://www.ebi.ac.uk/pdbsum/2hmt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hmt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KTRA_BACSU KTRA_BACSU] Catalytic subunit of the KtrAB potassium uptake transporter. The 2 major potassium transporter complexes KtrAB and KtrCD confer resistance to both suddenly imposed and prolonged osmotic stress.<ref>PMID:12562800</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hm/2hmt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hmt ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The KtrAB ion transporter is a complex of the KtrB membrane protein and KtrA, an RCK domain. RCK domains regulate eukaryotic and prokaryotic membrane proteins involved in K(+) transport. Conflicting functional models have proposed two different oligomeric arrangements for RCK domains, tetramer versus octamer. Our results for the KtrAB RCK domain clearly show an octamer in solution and in the crystal. We determined the structure of this protein in three different octameric ring conformations that resemble the RCK-domain octamer observed in the MthK potassium channel but show striking differences in size and symmetry. We present experimental evidence for the association between one RCK octameric ring and two KtrB membrane proteins. These results provide insights into the quaternary organization of the KtrAB transporter and its mechanism of activation and show that the RCK-domain octameric ring model is generally applicable to other ion-transport systems.
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==About this Structure==
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The RCK domain of the KtrAB K+ transporter: multiple conformations of an octameric ring.,Albright RA, Ibar JL, Kim CU, Gruner SM, Morais-Cabral JH Cell. 2006 Sep 22;126(6):1147-59. PMID:16990138<ref>PMID:16990138</ref>
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2HMT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=NAI:'>NAI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HMT OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The RCK domain of the KtrAB K+ transporter: multiple conformations of an octameric ring., Albright RA, Ibar JL, Kim CU, Gruner SM, Morais-Cabral JH, Cell. 2006 Sep 22;126(6):1147-59. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16990138 16990138]
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</div>
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<div class="pdbe-citations 2hmt" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Albright, R.A.]]
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[[Category: Albright RA]]
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[[Category: Morais-Cabral, J.H.]]
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[[Category: Morais-Cabral JH]]
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[[Category: NAI]]
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[[Category: ion transporter]]
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[[Category: ktn]]
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[[Category: ktr]]
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[[Category: ktra]]
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[[Category: ktrab]]
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[[Category: membrane protein]]
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[[Category: rck]]
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[[Category: symporter]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:24:23 2008''
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Current revision

Diamond-shaped octameric ring structure of an RCK domain with NADH bound

PDB ID 2hmt

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