2hua

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(New page: 200px<br /><applet load="2hua" size="350" color="white" frame="true" align="right" spinBox="true" caption="2hua" /> '''Solution Structure of CSFV IRES Domain IIa''...)
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[[Image:2hua.gif|left|200px]]<br /><applet load="2hua" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hua" />
 
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'''Solution Structure of CSFV IRES Domain IIa'''<br />
 
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==Overview==
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==Solution Structure of CSFV IRES Domain IIa==
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Internal ribosome entry site (IRES) RNAs from the hepatitis C virus (HCV), and classical swine fever virus (CSFV) coordinate cap-independent assembly, of eukaryotic 48S initiation complexes, consisting of the 40S ribosomal, subunit, eukaryotic initiation factor (eIF) 3 and the, eIF2/GTP/Met-tRNA(i)(Met) ternary complex. Here, we report that these, IRESes also play a functional role during 80S ribosome assembly downstream, of 48S complex formation, in promoting eIF5-induced GTP hydrolysis and, eIF2/GDP release from the initiation complex. We show that this function, is encoded in their independently folded IRES domain II and that it, depends both on its characteristic bent conformation and two conserved RNA, motifs, an apical hairpin loop and a loop E. Our data suggest a general, mode of subunit joining in HCV and HCV-like IRESes.
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<StructureSection load='2hua' size='340' side='right'caption='[[2hua]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hua]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HUA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hua OCA], [https://pdbe.org/2hua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hua RCSB], [https://www.ebi.ac.uk/pdbsum/2hua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hua ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Internal ribosome entry site (IRES) RNAs from the hepatitis C virus (HCV) and classical swine fever virus (CSFV) coordinate cap-independent assembly of eukaryotic 48S initiation complexes, consisting of the 40S ribosomal subunit, eukaryotic initiation factor (eIF) 3 and the eIF2/GTP/Met-tRNA(i)(Met) ternary complex. Here, we report that these IRESes also play a functional role during 80S ribosome assembly downstream of 48S complex formation, in promoting eIF5-induced GTP hydrolysis and eIF2/GDP release from the initiation complex. We show that this function is encoded in their independently folded IRES domain II and that it depends both on its characteristic bent conformation and two conserved RNA motifs, an apical hairpin loop and a loop E. Our data suggest a general mode of subunit joining in HCV and HCV-like IRESes.
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==About this Structure==
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HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly.,Locker N, Easton LE, Lukavsky PJ EMBO J. 2007 Feb 7;26(3):795-805. Epub 2007 Jan 25. PMID:17255934<ref>PMID:17255934</ref>
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2HUA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HUA OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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HCV and CSFV IRES domain II mediate eIF2 release during 80S ribosome assembly., Locker N, Easton LE, Lukavsky PJ, EMBO J. 2007 Feb 7;26(3):795-805. Epub 2007 Jan 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17255934 17255934]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 2hua" style="background-color:#fffaf0;"></div>
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[[Category: Easton, L.E.]]
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== References ==
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[[Category: Locker, N.]]
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<references/>
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[[Category: Lukavsky, P.J.]]
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__TOC__
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[[Category: rna hairpin]]
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</StructureSection>
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[[Category: Large Structures]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:28:57 2008''
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[[Category: Easton LE]]
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[[Category: Locker N]]
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[[Category: Lukavsky PJ]]

Current revision

Solution Structure of CSFV IRES Domain IIa

PDB ID 2hua

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