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2xkx

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[[Image:2xkx.jpg|left|200px]]
 
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==Single particle analysis of PSD-95 in negative stain==
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The line below this paragraph, containing "STRUCTURE_2xkx", creates the "Structure Box" on the page.
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<SX load='2xkx' size='340' side='right' viewer='molstar' caption='[[2xkx]], [[Resolution|resolution]] 22.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xkx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XKX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy , Hybrid , X-ray solution scattering, [[Resolution|Resolution]] 22.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xkx OCA], [https://pdbe.org/2xkx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xkx RCSB], [https://www.ebi.ac.uk/pdbsum/2xkx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xkx ProSAT]</span></td></tr>
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{{STRUCTURE_2xkx| PDB=2xkx | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DLG4_RAT DLG4_RAT] Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.<ref>PMID:15317815</ref> <ref>PMID:15358863</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The interaction of the extra-membranous domain of tetrameric inwardly rectifying Kir2.1 ion channels (Kir2.1NC(4)) with the membrane associated guanylate kinase protein PSD-95 has been studied using Transmission Electron Microscopy in negative stain. Three types of complexes were observed in electron micrographs corresponding to a 1:1 complex, a large self-enclosed tetrad complex and extended chains of linked channel domains. Using models derived from small angle X-ray scattering experiments in which high resolution structures from X-ray crystallographic and Nuclear Magnetic Resonance studies are positioned, the envelopes from single particle analysis can be resolved as a Kir2.1NC(4):PSD-95 complex and a tetrad of this unit (Kir2.1NC(4):PSD-95)(4). The tetrad complex shows the close association of the Kir2.1 cytoplasmic domains and the influence of PSD-95 mediated self-assembly on the clustering of these channels.
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===SINGLE PARTICLE ANALYSIS OF PSD-95 IN NEGATIVE STAIN===
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Self-directed assembly and clustering of the cytoplasmic domains of inwardly rectifying Kir2.1 potassium channels on association with PSD-95.,Fomina S, Howard TD, Sleator OK, Golovanova M, O'Ryan L, Leyland ML, Grossmann JG, Collins RF, Prince SM Biochim Biophys Acta. 2011 Jul 5;1808(10):2374-2389. PMID:21756874<ref>PMID:21756874</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2xkx" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12614619}}, adds the Publication Abstract to the page
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*[[Postsynaptic density protein|Postsynaptic density protein]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12614619 is the PubMed ID number.
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*[[Postsynaptic density protein 3D structures|Postsynaptic density protein 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_12614619}}
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<references/>
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__TOC__
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==About this Structure==
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</SX>
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[[2xkx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermomicrobium_roseum Thermomicrobium roseum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKX OCA].
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[[Category: Large Structures]]
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[[Category: Rattus norvegicus]]
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==Reference==
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[[Category: Collins RF]]
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<ref group="xtra">PMID:012614619</ref><ref group="xtra">PMID:010623522</ref><ref group="xtra">PMID:011779504</ref><references group="xtra"/>
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[[Category: Fomina S]]
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[[Category: Thermomicrobium roseum]]
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[[Category: Golovanova M]]
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[[Category: Collins, R F.]]
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[[Category: Grossmann JG]]
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[[Category: Fomina, S.]]
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[[Category: Howard TD]]
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[[Category: Golovanova, M.]]
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[[Category: Leyland ML]]
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[[Category: Grossmann, J G.]]
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[[Category: O'Ryan L]]
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[[Category: Howard, T D.]]
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[[Category: Prince SM]]
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[[Category: Leyland, M L.]]
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[[Category: Sleator OK]]
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[[Category: Prince, S M.]]
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[[Category: Ryan, L O.]]
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[[Category: Sleator, O K.]]
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[[Category: Membrane associated guanylate kinase]]
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[[Category: Scaffold protein]]
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[[Category: Structural protein]]
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Current revision

Single particle analysis of PSD-95 in negative stain

2xkx, resolution 22.90Å

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