3ahs

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[[Image:3ahs.png|left|200px]]
 
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==Crystal Structure of Ustilago sphaerogena Ribonuclease U2B==
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The line below this paragraph, containing "STRUCTURE_3ahs", creates the "Structure Box" on the page.
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<StructureSection load='3ahs' size='340' side='right'caption='[[3ahs]], [[Resolution|resolution]] 1.32&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ahs]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Ustilago_sphaerogena Ustilago sphaerogena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AHS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AHS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.32&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IAS:BETA-L-ASPARTIC+ACID'>IAS</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_3ahs| PDB=3ahs | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ahs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ahs OCA], [https://pdbe.org/3ahs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ahs RCSB], [https://www.ebi.ac.uk/pdbsum/3ahs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ahs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNU2_USTSP RNU2_USTSP]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ah/3ahs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ahs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Under physiological conditions, the deamidation and isomerization of asparagine to isoaspartate (isoAsp) proceeds non-enzymatically via succinimide. Although a large number of proteins have been reported to contain isoAsp, information concerning the three-dimensional structure of proteins containing isoaspartate is still limited. We have crystallized isoAsp containing Ustilago sphaerogena ribonuclease U2B, and determined the crystal structure at 1.32 A resolution. The structure revealed that the formation of isoAsp32 induces a single turn unfolding of the alpha-helix from Asp29 to Asp34, and the region from Asp29 to Arg35 forms a U-shaped loop structure. The electron density map shows that isoAsp32 retained the l-configuration at the C(alpha) atom. IsoAsp32 is in gauche conformation about a C(alpha)-C(beta) bond, and the polypeptide chain bends by approximately 90 degrees at isoAsp32. IsoAsp32 protrudes from the surface of the protein, and the abnormal beta-peptide bond in the main-chain and alpha-carboxylate in the side-chain is fully exposed. The structure suggests that the deamidation of the Asn and the isoAsp formation in proteins could confer immunogenicity. (c) 2010 Wiley Periodicals, Inc. Biopolymers, 2010.
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===Crystal Structure of Ustilago sphaerogena Ribonuclease U2B===
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Structural changes induced by the deamidation and isomerization of asparagine revealed by the crystal structure of Ustilago sphaerogena ribonuclease U2B.,Noguchi S Biopolymers. 2010 Jul 8. PMID:20623666<ref>PMID:20623666</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ahs" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20623666 is the PubMed ID number.
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{{ABSTRACT_PUBMED_20623666}}
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==About this Structure==
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[[3ahs]] is a 3 chain structure of [[Ribonuclease]] with sequence from [http://en.wikipedia.org/wiki/Ustilago_sphaerogena Ustilago sphaerogena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AHS OCA].
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==See Also==
==See Also==
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*[[Ribonuclease]]
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020623666</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Ustilago sphaerogena]]
[[Category: Ustilago sphaerogena]]
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[[Category: Noguchi, S.]]
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[[Category: Noguchi S]]
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[[Category: Hydrolase]]
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[[Category: Isoaspartate]]
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[[Category: Purine-specific endo-ribonuclease]]
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Current revision

Crystal Structure of Ustilago sphaerogena Ribonuclease U2B

PDB ID 3ahs

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