2igc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2igc" [edit=sysop:move=sysop])
Current revision (01:03, 21 November 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2igc.png|left|200px]]
 
-
<!--
+
==Structure of Spin labeled T4 Lysozyme Mutant T115R1A==
-
The line below this paragraph, containing "STRUCTURE_2igc", creates the "Structure Box" on the page.
+
<StructureSection load='2igc' size='340' side='right'caption='[[2igc]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2igc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IGC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IGC FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MTN:S-[(1-OXYL-2,2,5,5-TETRAMETHYL-2,5-DIHYDRO-1H-PYRROL-3-YL)METHYL]+METHANESULFONOTHIOATE'>MTN</scene></td></tr>
-
{{STRUCTURE_2igc| PDB=2igc | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2igc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2igc OCA], [https://pdbe.org/2igc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2igc RCSB], [https://www.ebi.ac.uk/pdbsum/2igc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2igc ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ig/2igc_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2igc ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
A nitroxide side chain (R1) has been substituted at single sites along a helix-turn-helix motif in T4 lysozyme (residues 114-135). Together with previously published data, the new sites reported complete a continuous scan through the motif. Mutants with R1 at sites 115 and 118 were selected for crystallographic analysis to identify the structural origins of the corresponding two-component EPR spectra. At 115, R1 is shown to occupy two rotamers in the room temperature crystal structure, one of which has not been previously reported. The two components in the EPR spectrum apparently arise from differential interactions of the two rotamers with the surrounding structure, the most important of which is a hydrophobic interaction of the nitroxide ring. Interestingly, the crystal structure at 100 K reveals a single rotamer, emphasizing the possibility of rotamer selection in low-temperature crystal structures. Residue 118 is at a solvent-inaccessible site in the protein core, and the structure of 118R1, the first reported for the R1 side chain at a buried site, reveals how the side chain is accommodated in an overpacked core.
-
===Structure of Spin labeled T4 Lysozyme Mutant T115R1A===
+
Structural determinants of nitroxide motion in spin-labeled proteins: tertiary contact and solvent-inaccessible sites in helix G of T4 lysozyme.,Guo Z, Cascio D, Hideg K, Kalai T, Hubbell WL Protein Sci. 2007 Jun;16(6):1069-86. Epub 2007 May 1. PMID:17473014<ref>PMID:17473014</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_17473014}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2igc" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 17473014 is the PubMed ID number.
+
-
-->
+
-
{{ABSTRACT_PUBMED_17473014}}
+
-
 
+
-
==About this Structure==
+
-
[[2igc]] is a 1 chain structure of [[Hen Egg-White (HEW) Lysozyme]] with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IGC OCA].
+
==See Also==
==See Also==
-
*[[Hen Egg-White (HEW) Lysozyme]]
+
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:017473014</ref><references group="xtra"/>
+
__TOC__
-
[[Category: Enterobacteria phage t4]]
+
</StructureSection>
-
[[Category: Lysozyme]]
+
[[Category: Escherichia virus T4]]
-
[[Category: Cascio, D.]]
+
[[Category: Large Structures]]
-
[[Category: Guo, Z.]]
+
[[Category: Cascio D]]
-
[[Category: Hideg, K.]]
+
[[Category: Guo Z]]
-
[[Category: Hubbell, W L.]]
+
[[Category: Hideg K]]
-
[[Category: Epr]]
+
[[Category: Hubbell WL]]
-
[[Category: Hydrolase]]
+
-
[[Category: Nitroxide]]
+
-
[[Category: Spin label]]
+
-
[[Category: T4 lysozyme]]
+

Current revision

Structure of Spin labeled T4 Lysozyme Mutant T115R1A

PDB ID 2igc

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2igc"
Personal tools