3h5r

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[[Image:3h5r.png|left|200px]]
 
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==Crystal structure of E. coli MccB + Succinimide==
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The line below this paragraph, containing "STRUCTURE_3h5r", creates the "Structure Box" on the page.
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<StructureSection load='3h5r' size='340' side='right'caption='[[3h5r]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3h5r]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H5R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H5R FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SNN:L-3-AMINOSUCCINIMIDE'>SNN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3h5r| PDB=3h5r | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h5r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h5r OCA], [https://pdbe.org/3h5r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h5r RCSB], [https://www.ebi.ac.uk/pdbsum/3h5r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h5r ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q47506_ECOLX Q47506_ECOLX]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h5/3h5r_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3h5r ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 39-kDa Escherichia coli enzyme MccB catalyses a remarkable posttranslational modification of the MccA heptapeptide during the biosynthesis of microcin C7 (MccC7), a 'Trojan horse' antibiotic. The approximately 260-residue C-terminal region of MccB is homologous to ubiquitin-like protein (UBL) activating enzyme (E1) adenylation domains. Accordingly, MccB-catalysed C-terminal MccA-acyl-adenylation is reminiscent of the E1-catalysed activation reaction. However, unlike E1 substrates, which are UBLs with a C-terminal di-glycine sequence, MccB's substrate, MccA, is a short peptide with an essential C-terminal Asn. Furthermore, after an intramolecular rearrangement of MccA-acyl-adenylate, MccB catalyses a second, unique reaction, producing a stable phosphoramidate-linked analogue of acyl-adenylated aspartic acid. We report six-crystal structures of MccB in apo, substrate-, intermediate-, and inhibitor-bound forms. Structural and kinetic analyses reveal a novel-peptide clamping mechanism for MccB binding to heptapeptide substrates and a dynamic-active site for catalysing dual adenosine triphosphate-consuming reactions. The results provide insight into how a distinctive member of the E1 superfamily carries out two-step activation for generating the peptidyl-antibiotic MccC7.
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===Crystal structure of E. coli MccB + Succinimide===
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How the MccB bacterial ancestor of ubiquitin E1 initiates biosynthesis of the microcin C7 antibiotic.,Regni CA, Roush RF, Miller DJ, Nourse A, Walsh CT, Schulman BA EMBO J. 2009 Jul 8;28(13):1953-64. Epub 2009 Jun 4. PMID:19494832<ref>PMID:19494832</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19494832}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3h5r" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19494832 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19494832}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3h5r]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H5R OCA].
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==Reference==
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<ref group="xtra">PMID:019494832</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Miller, D.]]
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[[Category: Large Structures]]
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[[Category: Nourse, A.]]
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[[Category: Miller D]]
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[[Category: Regni, C A.]]
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[[Category: Nourse A]]
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[[Category: Roush, R F.]]
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[[Category: Regni CA]]
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[[Category: Schulman, B A.]]
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[[Category: Roush RF]]
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[[Category: Walsh, C T.]]
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[[Category: Schulman BA]]
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[[Category: Antibiotic]]
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[[Category: Walsh CT]]
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[[Category: Antimicrobial]]
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[[Category: Bacteriocin]]
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[[Category: Mcc7]]
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[[Category: Microcin]]
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[[Category: N-p bond formation]]
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[[Category: Peptide antibiotic]]
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[[Category: Phosphoprotein]]
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[[Category: Transferase]]
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[[Category: Transferase-antibiotic complex]]
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[[Category: Ubiquitin-activating enzyme]]
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Current revision

Crystal structure of E. coli MccB + Succinimide

PDB ID 3h5r

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