2y7s

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[[Image:2y7s.jpg|left|200px]]
 
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==Structure of a designed meningococcal antigen (factor H binding protein, mutant G1) inducing broad protective immunity==
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The line below this paragraph, containing "STRUCTURE_2y7s", creates the "Structure Box" on the page.
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<StructureSection load='2y7s' size='340' side='right'caption='[[2y7s]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2y7s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis_MC58 Neisseria meningitidis MC58]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y7S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y7S FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y7s OCA], [https://pdbe.org/2y7s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y7s RCSB], [https://www.ebi.ac.uk/pdbsum/2y7s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y7s ProSAT]</span></td></tr>
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{{STRUCTURE_2y7s| PDB=2y7s | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FHBP_NEIMB FHBP_NEIMB] A bacterial surface lipoprotein that binds host (human) complement factor H (fH, gene CFH), binding contributes to the avoidance of complement-mediated lysis by N.meningitidis. Binding of fH to the bacteria surface is independent of bacterial sialic acid moieties (PubMed:16751403). fH binding affinity is high enough that it may sequester plasma fH, depleting its circulating levels and de-regulating complement in the host (Probable). This protein induces high levels of bactericidal antibodies in mice (PubMed:12642606, PubMed:15039331, PubMed:15664958, PubMed:21753121, PubMed:23133374).<ref>PMID:12642606</ref> <ref>PMID:15039331</ref> <ref>PMID:15664958</ref> <ref>PMID:16751403</ref> <ref>PMID:21753121</ref> <ref>PMID:23133374</ref> <ref>PMID:19225461</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The sequence variability of protective antigens is a major challenge to the development of vaccines. For Neisseria meningitidis, the bacterial pathogen that causes meningitis, the amino acid sequence of the protective antigen factor H binding protein (fHBP) has more than 300 variations. These sequence differences can be classified into three distinct groups of antigenic variants that do not induce cross-protective immunity. Our goal was to generate a single antigen that would induce immunity against all known sequence variants of N. meningitidis. To achieve this, we rationally designed, expressed, and purified 54 different mutants of fHBP and tested them in mice for the induction of protective immunity. We identified and determined the crystal structure of a lead chimeric antigen that was able to induce high levels of cross-protective antibodies in mice against all variant strains tested. The new fHBP antigen had a conserved backbone that carried an engineered surface containing specificities for all three variant groups. We demonstrate that the structure-based design of multiple immunodominant antigenic surfaces on a single protein scaffold is possible and represents an effective way to create broadly protective vaccines.
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===STRUCTURE OF A DESIGNED MENINGOCOCCAL ANTIGEN (FACTOR H BINDING PROTEIN, MUTANT G1) INDUCING BROAD PROTECTIVE IMMUNITY===
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Rational design of a meningococcal antigen inducing broad protective immunity.,Scarselli M, Arico B, Brunelli B, Savino S, Di Marcello F, Palumbo E, Veggi D, Ciucchi L, Cartocci E, Bottomley MJ, Malito E, Lo Surdo P, Comanducci M, Giuliani MM, Cantini F, Dragonetti S, Colaprico A, Doro F, Giannetti P, Pallaoro M, Brogioni B, Tontini M, Hilleringmann M, Nardi-Dei V, Banci L, Pizza M, Rappuoli R Sci Transl Med. 2011 Jul 13;3(91):91ra62. PMID:21753121<ref>PMID:21753121</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2y7s" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21753121 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21753121}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[2y7s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Neisseria_meningitidis_serogroup_b Neisseria meningitidis serogroup b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y7S OCA].
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[[Category: Neisseria meningitidis MC58]]
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[[Category: Bottomley MJ]]
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==Reference==
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[[Category: Malito E]]
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<ref group="xtra">PMID:021753121</ref><references group="xtra"/>
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[[Category: Spraggon G]]
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[[Category: Neisseria meningitidis serogroup b]]
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[[Category: Bottomley, M J.]]
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[[Category: Malito, E.]]
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[[Category: Spraggon, G.]]
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[[Category: Antibody]]
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[[Category: Antigen]]
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[[Category: Epitope]]
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[[Category: Immune system]]
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[[Category: Structure-based design]]
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[[Category: Vaccine]]
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Current revision

Structure of a designed meningococcal antigen (factor H binding protein, mutant G1) inducing broad protective immunity

PDB ID 2y7s

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