2j9i

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2j9i" size="350" color="white" frame="true" align="right" spinBox="true" caption="2j9i" /> '''LENGSIN IS A SURVIVOR OF AN ANCIENT FAMILY O...)
Current revision (09:35, 9 May 2024) (edit) (undo)
 
(19 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2j9i.jpg|left|200px]]<br /><applet load="2j9i" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2j9i" />
 
-
'''LENGSIN IS A SURVIVOR OF AN ANCIENT FAMILY OF CLASS I GLUTAMINE SYNTHETASES IN EUKARYOTES THAT HAS UNDERGONE EVOLUTIONARY RE-ENGINEERING FOR A TISSUE-SPECIFIC ROLE IN THE VERTEBRATE EYE LENS.'''<br />
 
-
==Overview==
+
==Lengsin is a survivor of an ancient family of class I glutamine synthetases in eukaryotes that has undergone evolutionary re- engineering for a tissue-specific role in the vertebrate eye lens.==
-
Lengsin is a major protein of the vertebrate eye lens. It belongs to the, hitherto purely prokaryotic GS I branch of the glutamine synthetase (GS), superfamily, but has no enzyme activity. Like the taxon-specific, crystallins, Lengsin is the result of the recruitment of an ancient enzyme, to a noncatalytic role in the vertebrate lens. Cryo-EM and modeling, studies of Lengsin show a dodecamer structure with important similarities, and differences with prokaryotic GS I structures. GS homology regions of, Lengsin are well conserved, but the N-terminal domain shows evidence of, dynamic evolutionary changes. Compared with birds and fish, most mammals, have an additional exon corresponding to part of the N-terminal domain;, however, in human, this is a nonfunctional pseudoexon. Genes related to, Lengsin are also present in the sea urchin, suggesting that this branch of, the GS I family, supplanted by GS II enzymes in vertebrates, has an, ancient role in metazoans.
+
<SX load='2j9i' size='340' side='right' viewer='molstar' caption='[[2j9i]], [[Resolution|resolution]] 17.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2j9i]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J9I FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 17&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j9i OCA], [https://pdbe.org/2j9i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j9i RCSB], [https://www.ebi.ac.uk/pdbsum/2j9i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j9i ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/LGSN_MOUSE LGSN_MOUSE] May act as a component of the cytoskeleton or as a chaperone for the reorganization of intermediate filament proteins during terminal differentiation in the lens. Does not seem to have enzymatic activity.<ref>PMID:18178558</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j9/2j9i_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j9i ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Lengsin is a major protein of the vertebrate eye lens. It belongs to the hitherto purely prokaryotic GS I branch of the glutamine synthetase (GS) superfamily, but has no enzyme activity. Like the taxon-specific crystallins, Lengsin is the result of the recruitment of an ancient enzyme to a noncatalytic role in the vertebrate lens. Cryo-EM and modeling studies of Lengsin show a dodecamer structure with important similarities and differences with prokaryotic GS I structures. GS homology regions of Lengsin are well conserved, but the N-terminal domain shows evidence of dynamic evolutionary changes. Compared with birds and fish, most mammals have an additional exon corresponding to part of the N-terminal domain; however, in human, this is a nonfunctional pseudoexon. Genes related to Lengsin are also present in the sea urchin, suggesting that this branch of the GS I family, supplanted by GS II enzymes in vertebrates, has an ancient role in metazoans.
-
==About this Structure==
+
Lengsin is a survivor of an ancient family of class I glutamine synthetases re-engineered by evolution for a role in the vertebrate lens.,Wyatt K, White HE, Wang L, Bateman OA, Slingsby C, Orlova EV, Wistow G Structure. 2006 Dec;14(12):1823-34. PMID:17161372<ref>PMID:17161372</ref>
-
2J9I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J9I OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Lengsin is a survivor of an ancient family of class I glutamine synthetases re-engineered by evolution for a role in the vertebrate lens., Wyatt K, White HE, Wang L, Bateman OA, Slingsby C, Orlova EV, Wistow G, Structure. 2006 Dec;14(12):1823-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17161372 17161372]
+
</div>
-
[[Category: Mus musculus]]
+
<div class="pdbe-citations 2j9i" style="background-color:#fffaf0;"></div>
-
[[Category: Single protein]]
+
-
[[Category: Bateman, O.A.]]
+
-
[[Category: Orlova, E.V.]]
+
-
[[Category: Slingsby, C.]]
+
-
[[Category: Wang, L.]]
+
-
[[Category: White, H.E.]]
+
-
[[Category: Wistow, G.]]
+
-
[[Category: Wyatt, K.]]
+
-
[[Category: ligase]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:52:02 2008''
+
==See Also==
 +
*[[Glutamine synthetase 3D structures|Glutamine synthetase 3D structures]]
 +
*[[Lengsin|Lengsin]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</SX>
 +
[[Category: Large Structures]]
 +
[[Category: Mus musculus]]
 +
[[Category: Bateman OA]]
 +
[[Category: Orlova EV]]
 +
[[Category: Slingsby C]]
 +
[[Category: Wang L]]
 +
[[Category: White HE]]
 +
[[Category: Wistow G]]
 +
[[Category: Wyatt K]]

Current revision

Lengsin is a survivor of an ancient family of class I glutamine synthetases in eukaryotes that has undergone evolutionary re- engineering for a tissue-specific role in the vertebrate eye lens.

2j9i, resolution 17.00Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2j9i"
Personal tools