2y0r

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[[Image:2y0r.png|left|200px]]
 
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==Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2==
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The line below this paragraph, containing "STRUCTURE_2y0r", creates the "Structure Box" on the page.
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<StructureSection load='2y0r' size='340' side='right'caption='[[2y0r]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2y0r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y0R FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y0r OCA], [https://pdbe.org/2y0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y0r RCSB], [https://www.ebi.ac.uk/pdbsum/2y0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y0r ProSAT]</span></td></tr>
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{{STRUCTURE_2y0r| PDB=2y0r | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MYS2_DICDI MYS2_DICDI] Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The cold-sensitive single-residue mutation of glycine 680 in the reactive thiol region of Dictyostelium discoideum myosin-2 or the corresponding conserved glycine in other myosin isoforms has been reported to interfere with motor function. Here we present the x-ray structures of myosin motor domain mutants G680A in the absence and presence of nucleotide as well as the apo structure of mutant G680V. Our results show that the Gly-680 mutations lead to uncoupling of the reactive thiol region from the surrounding structural elements. Structural and functional data indicate that the mutations induce the preferential population of a state that resembles the ADP-bound state. Moreover, the Gly-680 mutants display greatly reduced dynamic properties, which appear to be related to the recovery of myosin motor function at elevated temperatures.
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===STRUCTURAL BASIS FOR THE ALLOSTERIC INTERFERENCE OF MYOSIN FUNCTION BY MUTANTS G680A AND G680V OF DICTYOSTELIUM MYOSIN-2===
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Structural Basis for the Allosteric Interference of Myosin Function by Reactive Thiol Region Mutations G680A and G680V.,Preller M, Bauer S, Adamek N, Fujita-Becker S, Fedorov R, Geeves MA, Manstein DJ J Biol Chem. 2011 Oct 7;286(40):35051-60. Epub 2011 Aug 13. PMID:21841195<ref>PMID:21841195</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2y0r" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[2y0r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermomicrobium_roseum Thermomicrobium roseum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y0R OCA].
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*[[Myosin 3D Structures|Myosin 3D Structures]]
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[[Category: Thermomicrobium roseum]]
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== References ==
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[[Category: Adamek, N.]]
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<references/>
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[[Category: Bauer, S.]]
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__TOC__
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[[Category: Fedorov, R.]]
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</StructureSection>
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[[Category: Fujita-Becker, S.]]
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[[Category: Dictyostelium discoideum]]
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[[Category: Geeves, M A.]]
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[[Category: Large Structures]]
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[[Category: Manstein, D J.]]
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[[Category: Adamek N]]
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[[Category: Preller, M.]]
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[[Category: Bauer S]]
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[[Category: Motor protein]]
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[[Category: Fedorov R]]
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[[Category: Fujita-Becker S]]
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[[Category: Geeves MA]]
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[[Category: Manstein DJ]]
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[[Category: Preller M]]

Current revision

Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2

PDB ID 2y0r

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