2ns6

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(New page: 200px<br /><applet load="2ns6" size="350" color="white" frame="true" align="right" spinBox="true" caption="2ns6, resolution 2.100&Aring;" /> '''Crystal Structure o...)
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[[Image:2ns6.jpg|left|200px]]<br /><applet load="2ns6" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ns6, resolution 2.100&Aring;" />
 
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'''Crystal Structure of the Minimal Relaxase Domain of MobA from Plasmid R1162'''<br />
 
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==Overview==
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==Crystal Structure of the Minimal Relaxase Domain of MobA from Plasmid R1162==
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The plasmid R1162 encodes proteins that enable its conjugative, mobilization between bacterial cells. It can transfer between many, different species and is one of the most promiscuous of the mobilizable, plasmids. The plasmid-encoded protein MobA, which has both nicking and, priming activities on single-stranded DNA, is essential for mobilization., The nicking, or relaxase, activity has been localized to the 186 residue, N-terminal domain, called minMobA. We present here the 2.1 A X-ray, structure of minMobA. The fold is similar to that seen for two other, relaxases, TraI and TrwC. The similarity in fold, and action, suggests, these enzymes are evolutionary homologs, despite the lack of any, significant amino acid similarity. MinMobA has a well- defined target DNA, called oriT. The active site metal is observed near Tyr25, which is known, to form a phosphotyrosine adduct with the substrate. A model of the oriT, substrate complexed with minMobA has been made, based on observed, substrate binding to TrwC and TraI. The model is consistent with, observations of substrate base specificity, and provides a rationalization, for elements of the likely enzyme mechanism.
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<StructureSection load='2ns6' size='340' side='right'caption='[[2ns6]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ns6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NS6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ns6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ns6 OCA], [https://pdbe.org/2ns6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ns6 RCSB], [https://www.ebi.ac.uk/pdbsum/2ns6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ns6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/D0VWX2_PSEAI D0VWX2_PSEAI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ns/2ns6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ns6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The plasmid R1162 encodes proteins that enable its conjugative mobilization between bacterial cells. It can transfer between many different species and is one of the most promiscuous of the mobilizable plasmids. The plasmid-encoded protein MobA, which has both nicking and priming activities on single-stranded DNA, is essential for mobilization. The nicking, or relaxase, activity has been localized to the 186 residue N-terminal domain, called minMobA. We present here the 2.1 A X-ray structure of minMobA. The fold is similar to that seen for two other relaxases, TraI and TrwC. The similarity in fold, and action, suggests these enzymes are evolutionary homologs, despite the lack of any significant amino acid similarity. MinMobA has a well- defined target DNA called oriT. The active site metal is observed near Tyr25, which is known to form a phosphotyrosine adduct with the substrate. A model of the oriT substrate complexed with minMobA has been made, based on observed substrate binding to TrwC and TraI. The model is consistent with observations of substrate base specificity, and provides a rationalization for elements of the likely enzyme mechanism.
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==About this Structure==
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The structure of the minimal relaxase domain of MobA at 2.1 A resolution.,Monzingo AF, Ozburn A, Xia S, Meyer RJ, Robertus JD J Mol Biol. 2007 Feb 9;366(1):165-78. Epub 2006 Nov 11. PMID:17157875<ref>PMID:17157875</ref>
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2NS6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The structure of the minimal relaxase domain of MobA at 2.1 A resolution., Monzingo AF, Ozburn A, Xia S, Meyer RJ, Robertus JD, J Mol Biol. 2007 Feb 9;366(1):165-78. Epub 2006 Nov 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17157875 17157875]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 2ns6" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Meyer, R.J.]]
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[[Category: Meyer RJ]]
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[[Category: Monzingo, A.F.]]
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[[Category: Monzingo AF]]
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[[Category: Ozburn, A.]]
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[[Category: Ozburn A]]
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[[Category: Robertus, J.D.]]
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[[Category: Robertus JD]]
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[[Category: Xia, S.]]
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[[Category: Xia S]]
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[[Category: MN]]
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[[Category: 5-strand antiparallel beta sheet]]
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[[Category: hydrolase]]
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[[Category: metalloenzyme]]
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[[Category: nickase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 20:58:54 2008''
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Current revision

Crystal Structure of the Minimal Relaxase Domain of MobA from Plasmid R1162

PDB ID 2ns6

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